Skip to main content

Advertisement

SpringerLink
Log in

Expression of transcripts for fibroblast growth factor 18 and its possible receptors during postnatal dentin formation in rat molars

  • Original Article
  • Published:
Odontology Aims and scope Submit manuscript

Abstract

Fibroblast growth factors (FGFs) regulate the proliferation and differentiation of various cells via their respective receptors (FGFRs). During the early stages of tooth development in fetal mice, FGFs and FGFRs have been shown to be expressed in dental epithelia and mesenchymal cells at the initial stages of odontogenesis and to regulate cell proliferation and differentiation. However, little is known about the expression patterns of FGFs in the advanced stages of tooth development. In the present study, we focused on FGF18 expression in the rat mandibular first molar (M1) during the postnatal crown and root formation stages. FGF18 signals by RT-PCR using cDNAs from M1 were very weak at postnatal day 5 and were significantly up-regulated at days 7, 9 and 15. Transcripts were undetectable by in situ hybridization (ISH) but could be detected by in situ RT-PCR in the differentiated odontoblasts and cells of the sub-odontoblastic layer in both crown and root portions of M1 at day 15. The transcripts of FGFR2c and FGFR3, possible candidate receptors of FGF18, were detected by RT-PCR and ISH in differentiated odontoblasts throughout postnatal development. These results suggest the continual involvement of FGF18 signaling in the regulation of odontoblasts during root formation where it may contribute to dentin matrix formation and/or mineralization.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Armelin HA. Pituitary extracts and steroid hormones in the control of 3T3 cell growth. Proc Nat Acad Sci USA. 1973;70:2702–6.

    Article  PubMed Central  PubMed  Google Scholar 

  2. Powers CJ, McLeskey SW, Wellstein A. Fibroblast growth factors, their receptors and signaling. Endocr Relat Cancer. 2000;7:165–97.

    Article  PubMed  Google Scholar 

  3. Bugler B, Amalric F, Prats H. Alternative initiation of translation determines cytoplasmic or nuclear localization of basic fibroblast growth factor. Mol Cell Biol. 1991;11:573–7.

    PubMed Central  PubMed  Google Scholar 

  4. Quarto N, Finger FP, Rifkin DB. The NH2-terminal extension of high molecular weight bFGF is a nuclear targeting signal. J Cell Physiol. 1991;147:311–8.

    Article  PubMed  Google Scholar 

  5. Imamura T, Tokita Y, Mitsui Y. Identification of a heparin-binding growth factor-1 nuclear translocation sequence by deletion mutation analysis. J Biol Chem. 1992;267:5676–9.

    PubMed  Google Scholar 

  6. Kettunen P, Thesleff I. Expression and function of FGFs-4, -8, and -9 suggest functional redundancy and repetitive use as epithelial signals during tooth morphogenesis. Dev Dyn. 1998;211:256–68.

    Article  PubMed  Google Scholar 

  7. Jernvall J, Kettunen P, Karavanova I, Martin LB, Thesleff I. Evidence for the role the enamel knot as a control center in mammalian tooth cusp formation: non-dividing cells express growth stimulating Fgf-4 gene. Int J Dev Biol. 1994;38:463–9.

    PubMed  Google Scholar 

  8. Orr-Urtreger A, Givol D, Yayon A, Yarden Y, Lonai P. Developmental expression of two murine fibroblast growth factor receptors, flg and bek. Development. 1991;113:1419–34.

    PubMed  Google Scholar 

  9. Peters KG, Werner S, Chen G, Williams LT. Two FGF receptor genes are differentially expressed in epithelial and mesenchymal tissues during limb formation and organogenesis in the mouse. Development. 1992;114:223–43.

    Google Scholar 

  10. Kettunen P, Karavanova I, Thesleff I. Responsiveness of developing dental tissues to fibroblast growth factors: expression of splicing alternatives of FGFR1, -2, -3, and of FGFR4; and stimulation of cell proliferation by FGF-2, -4, -8, and -9. Dev Genet. 1998;22:374–85.

    Article  PubMed  Google Scholar 

  11. Reinhold MI, Naski MC. Direct interactions of Runx2 and canonical Wnt signaling induce FGF18. J Biol Chem. 2007;282:3653–63.

    Article  PubMed  Google Scholar 

  12. Chen J, Lan Y, Baek J-A, Gao Y, Jiang R. Wnt/beta-catenin signaling plays an essential role in activation of odontogenic mesenchyme during early tooth development. Dev Biol. 2009;334:174–85.

    Article  PubMed Central  PubMed  Google Scholar 

  13. Yamashiro T, Zheng L, Shitaku Y, Saito M, Tsubakimoto T, Takada K, Takano-Yamamoto T, Thesleff I. Wnt10a regulates dentin sialophosphoprotein mRNA expression and possibly links odontoblast differentiation and tooth morphogenesis. Differentiation. 2007;75:452–62.

    Article  PubMed  Google Scholar 

  14. Kim T-H, Lee J-Y, Baek J-A, Lee J-C, Yang X, Taketo MM, Jiang R, Cho E-S. Consitutive stabilization of β-catenin in the dental mesenchyme leads to excessive dentin and cementum formation. Biochem Biophys Res Commun. 2011;412:549–55.

    Article  PubMed  Google Scholar 

  15. Peng L, Ren LB, Dong G, Wang CL, Xu P, Ye L, Zhou XD. Wnt5a promotes differentiation of human dental papilla cells. Int Endod J. 2010;43:404–12.

    Article  PubMed  Google Scholar 

  16. Ohbayashi N, Hoshikawa M, Kimura S, Yamasaki M, Fukui S, Itoh N. Structure and expression of the mRNA encoding a novel fibroblast growth factor, FGF18. J Biol Chem. 1998;29:18161–4.

    Article  Google Scholar 

  17. Itoh N, Ornitz DM. Evolution of the Fgf and Fgfr gene families. Trends Genet. 2004;20:563–9.

    Article  PubMed  Google Scholar 

  18. Maruoka Y, Ohbayashi N, Hoshikawa M, Itoh N, Hogan BLM, Furuta Y. Comparison of the expression of three highly related genes, Fgf8, Fgf17 and Fgf18, in the mouse embryo. Mech Dev. 1998;74:175–7.

    Article  PubMed  Google Scholar 

  19. Ohuchi H, Kimura S, Watamoto M, Itoh N. Involvement of fibroblast growth factor (FGF)18-FGF8 signaling in specification of left-right asymmetry and brain and limb development of the chick embryo. Mech Dev. 2000;95:55–66.

    Article  PubMed  Google Scholar 

  20. Ohbayashi N, Shibayama M, Kurotaki Y, Imanishi M, Fujimori T, Itoh N, Takada S. FGF18 is required for normal cell proliferation and differentiation during osteogenesis and chondrogenesis. Genes Dev. 2002;16:870–9.

    Article  PubMed Central  PubMed  Google Scholar 

  21. Wang H, Yoshiko Y, Yamamoto R, Minamizaki T, Kozai K, Tanne K, Aubin JE, Maeda N. Overexpression of fibroblast growth factor 23 suppresses osteoblast differentiation and matrix mineralization in vitro. J Bone Miner Res. 2008;23:939–48.

    Article  PubMed  Google Scholar 

  22. Nuovo GJ. PCR in situ Hybridization; Protocols and Applications. 2nd ed. New York: Raven Press; 1994. p. 247–306.

    Google Scholar 

  23. Baba O, Qin C, Brunn JC, Jones JE, Wygant JN, McIntyre BW, Butler WT. Detection of dentin sialoprotein in rat periodontium. Eur J Oral Sci. 2004;112:163–70.

    Article  PubMed  Google Scholar 

  24. Spann W, Pachmann K, Zabnienska H, Pielmeier A, Emmerich B. In situ amplification of single copy gene segments in individual cells by the polymerase chain reaction. Infection. 1991;19:242–4.

    Article  PubMed  Google Scholar 

  25. Chen RH, Fuggle SV. In situ cDNA polymerase chain reaction. A novel technique for detecting mRNA expression. Am J Pathol. 1993;143:1527–34.

    PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by a Grant-in-Aid for Scientific Research (to OB, TT) from the Japan Society for the Promotion of Science (#18591996), and by a Grant for Supporting Project for Strategic Research of Nihon University, School of Dentistry at Matsudo by the Ministry of Education, Culture, Sports, Science and Technology, 2008–2011 (Team: Dental morphogenesis; to OB, TT and MO).

Conflict of interest

All authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Section of Biology, Department of Oral Function and Molecular Biology, School of Dentistry, Ohu University, 31-1 Misumido, Tomita, Koriyama, Fukushima, 963-8611, Japan

    Otto Baba

  2. Division of Biostructural Science, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan

    Otto Baba, Makoto J. Tabata & Yoshiro Takano

  3. Department of Food and Nutrition, Faculty of Human Sciences and Design, Japan Women’s University, 2-8-1 Mejirodai, Bunkyo-ku, Tokyo, 112-8681, Japan

    Masato S. Ota

  4. Division of Molecular Craniofacial Embryology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan

    Masato S. Ota

  5. Division of Maxillofacial Anatomy, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan

    Tatsuo Terashima

Authors
  1. Otto Baba
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masato S. Ota
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tatsuo Terashima
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Makoto J. Tabata
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yoshiro Takano
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Otto Baba.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Baba, O., Ota, M.S., Terashima, T. et al. Expression of transcripts for fibroblast growth factor 18 and its possible receptors during postnatal dentin formation in rat molars. Odontology 103, 136–142 (2015). https://doi.org/10.1007/s10266-013-0147-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10266-013-0147-9

Keywords

Search

Navigation