Abstract
This study investigated the association between clinical and salivary or molecular parameters in Down syndrome subjects. Sixty individuals (1- to 48-year old) were clinically examined using DMFT/DMFS. Stimulated saliva was collected; salivary flow was calculated (mL/min), buffering capacity was measured using a standard pH tape. In addition, 25 μL of saliva was diluted using 10-fold-dilution method and then placed on Mitis-Salivarius-Bacitracin agar to count colony forming units (CFU/mL) of mutans streptococci. Polymerase chain reaction analysis identified species. Caries indexes were 0.65–13.5 (DMFT) and 0.65–26.0 (DMFS) according to groups. Ninety-four percent of subjects had low flow rate (0.7–1.0 mL/min) and 44% had low buffering capacity (pH < 4). Besides, 60% had more than 1 × 106 CFU/mL, 60% had S. mutans, and 41.4% had S. sobrinus. Caries indexes did not significantly correlate with flow rate, buffering capacity, CFU/mL by Pearson’s correlation (p > 0.05), and showed no significant association with prevalence of species by Chi-square (p > 0.05). There is no association between clinical picture and salivary or molecular parameters in Down syndrome subjects.
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The study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP-Grant 04/12925-0).
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de Castilho, A.R.F., Pardi, V. & Pereira, C.V. Dental caries experience in relation to salivary findings and molecular identification of S. mutans and S. sobrinus in subjects with Down syndrome. Odontology 99, 162–167 (2011). https://doi.org/10.1007/s10266-011-0010-9
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DOI: https://doi.org/10.1007/s10266-011-0010-9