Weak transgenerational effects of ancestral nitrogen and phosphorus availabilities on offspring phenotypes in Arabidopsis thaliana

Abstract

Nutrient availability significantly regulates plant growth and metabolic functions, but whether and how the long-term exposure of ancestral plants to contrasting nutrient environments influences offspring phenotypic performance (i.e., transgenerational plasticity) remain poorly addressed. Here we conducted experimental manipulations using Arabidopsis thaliana with the ancestral plants grown in different nitrogen (N) and phosphorus (P) availabilities over eleven consecutive generations, and then examined the offspring phenotypic performance under the interactive effects of current and ancestral nutrient environments. We found that current rather than ancestral nutrient environments dominantly explained the variations in offspring plant traits (i.e., flowering time, aboveground biomass and biomass allocation fractions), suggesting the relatively weak transgenerational effects of ancestral N and P availabilities on offspring phenotypes. In contrast, increasing N and P availabilities in the offspring generation remarkably shortened the flowering time, increased the aboveground biomass, and altered biomass allocation fractions differentially among organs. Despite the overall weak transgenerational phenotypic plasticity, under the low nutrient environment, the offspring of ancestral plants from the low nutrient environment had a significantly higher fruit mass fraction than those from the suitable nutrient environment. Taken together, our findings suggest that A. thaliana exhibits a much stronger within- than trans-generational trait plasticity under contrasting nutrient availabilities, and may provide important insights into the understanding of plant adaptation and evolutionary processes under changing nutrient environments.