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Complete genotyping in conservation genetics, a case study of a critically endangered shrub, Stachyurus macrocarpus var. prunifolius (Stachyuraceae) in the Ogasawara Islands, Japan

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Abstract

More than 3,000 species are listed as critically endangered worldwide, and various conservation measures such as habitat restoration, assisted reproduction and establishment of ex situ populations would be required to prevent their extinction. We determined the genotype of all 15 known wild clumps using nuclear microsatellite markers for Stachyurus macrocarpus var. prunifolius, a critically endangered shrub endemic to the Ogasawara (Bonin) Islands of Japan. In addition, the seedlings propagated from seeds taken from one wild clumps were genotyped. The results of complete genotyping showed that both wild and nursery populations had population-specific alleles. Two alleles were detected only in the nursery population, indicating the existence of undiscovered mature individuals in the wild. Four alleles were found only in the wild and were detected in two geographically isolated clumps, and this finding may propose that re-introduction and transplantation between different sites requires sensitive handling in terms of the conservation of evolutionary significant units. These results show that complete genotyping can provide essential genetic and ecological information for effective management of endangered species.

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Acknowledgments

We thank F. Shibazaki and Y. Hoshi for sampling. We also thank J. Worth for his helpful comments on drafts of this manuscript. This work was supported by Grants in Aid for Scientific Research (20241056) from the Japan Society for the Promotion of Science, and the Environment Research and Technology Development Fund (D-0903) of the Ministry of the Environment, Japan.

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Authors and Affiliations

  1. Graduate School of Symbiotic Systems Science and Technology, Fukushima University, 1 Kaneyagawa, Fukushima, 960-1296, Japan

    Shingo Kaneko

  2. Kyushu Research Center, Forestry and Forest Products Research Institute, 4-11-16 Kurokami, Kumamoto, 860-0862, Japan

    Tetsuto Abe

  3. Laboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan

    Yuji Isagi

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  1. Shingo Kaneko
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  2. Tetsuto Abe
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  3. Yuji Isagi
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Correspondence to Yuji Isagi.

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Kaneko, S., Abe, T. & Isagi, Y. Complete genotyping in conservation genetics, a case study of a critically endangered shrub, Stachyurus macrocarpus var. prunifolius (Stachyuraceae) in the Ogasawara Islands, Japan. J Plant Res 126, 635–642 (2013). https://doi.org/10.1007/s10265-013-0554-6

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  • DOI: https://doi.org/10.1007/s10265-013-0554-6

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