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Distribution pattern changes of actin filaments during chloroplast movement in Adiantum capillus-veneris

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Abstract

Chloroplasts change their positions in a cell in response to light intensities. The photoreceptors involved in chloroplast photo-relocation movements and the behavior of chloroplasts during their migration were identified in our previous studies, but the mechanism of movement has yet to be clarified. In this study, the behavior of actin filaments under various light conditions was observed in Adiantum capillus-veneris gametophytes. In chloroplasts staying in one place under a weak light condition and not moving, circular structures composed of actin filaments were observed around the chloroplast periphery. In contrast, short actin filaments were observed at the leading edge of moving chloroplasts induced by partial cell irradiation. In the dark, the circular structures found under the weak light condition disappeared and then reappeared around the moving chloroplasts. Mutant analyses revealed that the disappearance of the circular actin structure was mediated by the blue light photoreceptor, phototropin2.

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Acknowledgments

This work was partially supported by the Japanese Ministry of Education, Sports, Science and Technology (MEXT 17084006 to M.W.) and the Japan Society for the Promotion of Science (JSPS 16107002 and 20227001 to M.W.).

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  1. Kyushu University, Hakozaki 6-10-1, Fukuoka, 812-8581, Japan

    Hidenori Tsuboi & Masamitsu Wada

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  1. Hidenori Tsuboi
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  2. Masamitsu Wada
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Correspondence to Masamitsu Wada.

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Tsuboi, H., Wada, M. Distribution pattern changes of actin filaments during chloroplast movement in Adiantum capillus-veneris . J Plant Res 125, 417–428 (2012). https://doi.org/10.1007/s10265-011-0444-8

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