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Transcriptional control of the dihydroflavonol 4-reductase multigene family in Lotus japonicus

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Abstract

In the genome of the model legume Lotus japonicus, dihydroflavonol 4-reductase (DFR), which is the first committed enzyme of the anthocyanin and proanthocyanidin (PA) pathways, is encoded as a tandemly arrayed five-gene family. Expression analysis revealed that both organ specificity and stress responsiveness differ among the DFRs. To elucidate the regulatory mechanisms underlying the expression of DFRs, we investigated the transcriptional control of each member of the DFR multigene family. Ectopic expression of a combination of the transcription factors MYB, bHLH, and WDR showed that only the DFR2 promoter was activated, indicating that each member of the DFR gene family is regulated independently.

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Authors and Affiliations

  1. Department of Biological Sciences, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo, 112-8610, Japan

    Kazuko Yoshida, Rieko Iwasaka & Masaaki Sakuta

  2. Kazusa DNA Research Institute, Kazusa-Kamatari 2-6-7, Kisarazu, 292-0818, Japan

    Norimoto Shimada

  3. Department of Applied Biological Sciences, Nihon University, Fujisawa, Kanagawa, 252-8510, Japan

    Shin-ichi Ayabe & Toshio Aoki

Authors
  1. Kazuko Yoshida
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  2. Rieko Iwasaka
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  3. Norimoto Shimada
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  4. Shin-ichi Ayabe
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  5. Toshio Aoki
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  6. Masaaki Sakuta
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Correspondence to Masaaki Sakuta.

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K. Yoshida and R. Iwasaka contributed equally to this work.

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Yoshida, K., Iwasaka, R., Shimada, N. et al. Transcriptional control of the dihydroflavonol 4-reductase multigene family in Lotus japonicus . J Plant Res 123, 801–805 (2010). https://doi.org/10.1007/s10265-010-0325-6

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  • DOI: https://doi.org/10.1007/s10265-010-0325-6

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