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Recharacterization of Chlamydomonas reinhardtii and its relatives with new isolates from Japan

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Abstract

Chlamydomonas reinhardtii P. A. Dang. (Volvocales, Chlorophyceae) is one of the most intensely studied algae, and its whole genome was sequenced. Although this species was originally described based on materials from France and is often referred to as a cosmopolitan species, all culture strains available today have been isolated from eastern North America. The distinctions with similar and/or closely related species, such as Chlamydomonas globosa J. Snow and Chlamydomonas orbicularis E. G. Pringsh., are also contentious. In this study, new strains of C. reinhardtii and C. globosa were isolated from Japan and compared with several strains similar to C. reinhardtii. Based on the morphological, genealogical, phylogenetical, and mating studies including the new Japanese strains, the circumscription of C. reinhardtii was clarified. C. reinhardtii was most closely related to C. globosa, and they were shown to be different species. Although C. reinhardtii was similar to C. orbicularis, the authentic strain of C. orbicularis was morphologically distinguishable and phylogenetically distant from C. reinhardtii. Discovery of the Japanese strains of C. reinhardtii supports the cosmopolitan distribution of this species. Based on Japanese strains and/or strains from other countries, emended descriptions of C. reinhardtii, C. globosa, and C. orbicularis are given.

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Acknowledgments

We are grateful to Dr. Kazuyuki Miyaji (Toho University) for his kind help with the sample collection in Kagoshima. We thank all members of the Institute for Advanced Biological Sciences at Keio University for their kind support. This work was supported by grants from Yamagata Prefectural Government and Tsuruoka City.

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Authors and Affiliations

  1. Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan

    Takashi Nakada, Haruka Shinkawa, Takuro Ito & Masaru Tomita

  2. Graduate School of Media and Governance, Keio University, Fujisawa, 252-8520, Japan

    Takashi Nakada, Takuro Ito & Masaru Tomita

  3. Faculty of Environment and Information Studies, Keio University, Fujisawa, 252-8520, Japan

    Haruka Shinkawa & Masaru Tomita

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  1. Takashi Nakada
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  2. Haruka Shinkawa
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Correspondence to Takashi Nakada.

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Nakada, T., Shinkawa, H., Ito, T. et al. Recharacterization of Chlamydomonas reinhardtii and its relatives with new isolates from Japan. J Plant Res 123, 67–78 (2010). https://doi.org/10.1007/s10265-009-0266-0

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  • DOI: https://doi.org/10.1007/s10265-009-0266-0

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