Abstract
Genetic variation associated with Picea jezoensis populations of South Korea was investigated using chloroplast (cp), mitochondrial (mt) and nuclear DNA markers. In South Korea, P. jezoensis is distributed across a very restricted area, being found on the summits of three mountains: Mts. Jiri, Dokyu and Gyebang. Examination of five region restriction enzyme combinations for mtDNA and four for cpDNA revealed haplotypes endemic to South Korea. The Gyebang population, the most northerly and most isolated, was genetically distinct from the other populations. Nuclear microsatellite markers indicated, overall, a low level of genetic diversity (H e = 0.406) in South Korea; this could be attributed to genetic drift and/or founder effects associated with historical events. The Wilcoxon sign-rank test did not indicate a recent bottleneck in any of the populations irrespective of the model considered (infinite allele model, two-phased model of mutation, and stepwise mutation model). Microsatellite markers also demonstrated that the Gyebang population was distinct from the others. The results of this study could be used as the basis for conservation guidelines for the management of this species in South Korea.
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Acknowledgments
This study was supported by the post-doctoral fellowship program for foreign researchers run by the Korea Forest Research Institute. The authors thank S.-D. Hur, J.-J. Lee, J.-H. Song and B.-H. Yang for their help in collecting needles, and the Korea National Park Service for its cooperation in allowing us to collect samples from two national peaks: Dokyu and the Jiri mountains. We also thank M. Aizawa, Y. Tsumura and Y. Tsuda for their kind advice and valuable suggestions.
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Moriguchi, Y., Kang, KS., Lee, KY. et al. Genetic variation of Picea jezoensis populations in South Korea revealed by chloroplast, mitochondrial and nuclear DNA markers. J Plant Res 122, 153–160 (2009). https://doi.org/10.1007/s10265-008-0210-8
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DOI: https://doi.org/10.1007/s10265-008-0210-8