Abstract
The vascular tissue of roots performs essential roles in the physical support and transport of water, nutrients, and signaling molecules in higher plants. The molecular mechanisms underlying the function of root vascular tissue are poorly understood. In this study, we analyzed the expression pattern of AtGRP9, a salt stress-responsive gene encoding a glycine-rich protein, and its interacting partner, in Arabidopsis thaliana. Analysis of GUS or GFP expression under the control of the AtGRP9 promoter showed that AtGRP9 was expressed in the vascular tissue of the root; subcellular localization analysis further demonstrated that AtGRP9 proteins were localized in the cell wall and in the cytoplasm. Yeast two-hybrid analysis revealed that AtGRP9 interacted with AtCAD5, a major cinnamyl alcohol dehydrogenase (CAD) involved in lignin biosynthesis, for which tissue-specific distribution was comparable with that of AtGRP9. These results suggest that AtGRP9 may be involved in lignin synthesis in response to salt stress as a result of its interaction with AtCAD5 in A. thaliana.
References
Anderson CM, Wagner TA, Perret M, He ZH, He D, Kohorn BD (2001) WAKs: cell wall-associated kinases linking the cytoplasm to the extracellular matrix. Plant Mol Biol 47:197–206
Azaizeh H, Steudle E (1991) Effects of salinity on water transport of excised maize (Zea mays L.) roots. Plant Physiol 97:1136–1145
Condit CM, Meagher RB (1986) A gene encoding a novel glycine-rich structural protein of petunia. Nature 323:178–181
deOliveira DE, Seurinck J, Inzé D, Van Montagu M, Botterman J (1990) Differential expression of five Arabidopsis genes encoding glycine-rich proteins. Plant Cell 2:427–436
Fang RX, Pang Z, Gao DM, Mang KQ, Chua NH (1991) cDNA sequence of a virus-inducible, glycine-rich protein gene from rice. Plant Mol Biol 17:1255–1257
Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907
Keller B, Sauer N, Lamb CJ (1988) Glycine-rich cell wall proteins in bean: gene structure and association of the protein with the vascular system. EMBO J 7:3625–3633
Kim SJ, Kim MR, Bedgar DL, Moinuddin SG, Cardenas CL, Davin LB, Kang C, Lewis NG (2004) Functional reclassification of the putative cinnamyl alcohol dehydrogenase multigene family in Arabidopsis. Proc Natl Acad Sci USA 101:1455–1460
Matsuyama T, Satoh H, Yamada Y, Hashimoto T (1999) A maize glycine-rich protein is synthesized in the lateral root cap and accumulates in the mucilage. Plant Physiol 120:665–674
Park AR, Cho SK, Yun UJ, Jin MY, Lee SH, Sachetto-Martins G, Park OK (2001) Interaction of the Arabidopsis receptor protein kinase Wak1 with a glycine-rich protein, AtGRP-3. J Biol Chem 276:26688–26693
Ringli C, Keller B, Ryser U (2001) Glycine-rich proteins as structural components of plant cell walls. Cell Mol Life Sci 58:1430–1441
Rohde W, Rosch K, Kroger K, Salamini F (1990) Nucleotide sequence of a Hordeum vulgare gene encoding a glycine-rich protein with homology to vertebrate cytokeratins. Plant Mol Biol 14:1057–1059
Sachetto-Martins G, Franco LO, de Oliveira DE (2000) Plant glycine-rich proteins: a family or just proteins with a common motif? Biochim Biophys Acta 1492:1–14
Sakuta C, Satoh S (2000) Vascular tissue-specific gene expression of xylem sap glycine-rich proteins in roots and their localization in the walls of metaxylem vessels in cucumber. Plant Cell Physiol 41:627–638
Sanchez-Aguayo I, Rodriguez-Galan JM, Garcia R, Torreblanca J, Pardo JM (2004) Salt stress enhances xylem development and expression of S-adenosyl-L-methionine synthase in lignifying tissue of tomato plants. Planta 220:278–285
Scott A, Wyatt S, Tsou PL, Robertson D, Allen NS (1999) Model system for plant cell biology: GFP imaging in living onion epidermal cells. Biotechniques 26:1125, 1128–1132
Sibout R, Eudes A, Pollet B, Goujon T, Mila I, Granier F, Seguin A, Lapierre C, Jouanin L (2003) Expression pattern of two paralogs encoding cinnamyl alcohol dehydrogenases in Arabidopsis. Isolation and characterization of the corresponding mutants. Plant Physiol 132:848–860
Tang YX, Chen AP, Liu SG, Xia GX (2003) Identification and functional characterization of a salt tolerance-responsive gene (AtGRP9) of Arabidopsis. Prog Nat Sci 13:50–54
Ueki S, Citovsky V (2005) Identification of an interactor of cadmium ion-induced glycine-rich protein involved in regulation of callose levels in plant vasculature. Proc Natl Acad Sci USA 102:12089–12094
Xie Q, Guo HS, Dallman G, Fang S, Weissman AM, Chua NH (2002) SINAT5 promotes ubiquitin-related degradation of NAC1 to attenuate auxin signals. Nature 419:167–170
Acknowledgments
We would like to thank Dr Qi Xie (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) for providing the A. thaliana cDNA library. This work is supported by the National Foundation of Natural Sciences (Grant No: 30370327) and the Oriented Projects of the Chinese Academy of Sciences (No: KSCX2-YW-N-012).
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Chen, AP., Zhong, NQ., Qu, ZL. et al. Root and vascular tissue-specific expression of glycine-rich protein AtGRP9 and its interaction with AtCAD5, a cinnamyl alcohol dehydrogenase, in Arabidopsis thaliana . J Plant Res 120, 337–343 (2007). https://doi.org/10.1007/s10265-006-0058-8
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DOI: https://doi.org/10.1007/s10265-006-0058-8