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Molecular cloning and differential expression of an γ-aminobutyrate transaminase gene, OsGABA-T, in rice (Oryza sativa) leaves infected with blast fungus

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Abstract

γ-Aminobutyrate transaminase (GABA-T) catalyzes the conversion of GABA to succinic semialdehyde. Using differential display PCR and cDNA library screening, a full-length GABA-T cDNA (OsGABA-T) was isolated from rice (Oryza sativa) leaves infected with an incompatible race of Magnaporthe grisea. The deduced amino acid sequence comprises 483 amino acid residues and shares 85–69% identity with GABA-T sequences from other plants. OsGABA-T expression is induced by blast fungus infection, mechanical wounding and ultraviolet radiation in rice leaves and is not detected in normal rice organs. This gene is also induced by defense signal molecules such as salicylic acid and abscisic acid, but not by jasmonic acid. Our data suggest that OsGABA-T (GABA shunt) may play a role in restricting the levels of cell death during the host–pathogen interaction.

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Acknowledgement

This research was supported by a project from Yunnan Provincial Collaboration Program (Cloning and Functional Analysis of Rice Genes Induced by Magnaporthe grisea, no. 98ZN08)

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  1. Chunxia Wu

    Present address: Key Lab of Plant Stress Research, Life Science College, Shandong Normal University, Jinan, Shandong Province, People’s Republic of China

Authors and Affiliations

  1. Department of Plant Pathology, College of Agriculture and Biotechnology, China Agricultural University, Yuanmingyuan West Road No. 2, 100094, Beijing, People’s Republic of China

    Chunxia Wu, Shanyue Zhou, Quan Zhang, Wensheng Zhao & Youliang Peng

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Correspondence to Youliang Peng.

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Wu, C., Zhou, S., Zhang, Q. et al. Molecular cloning and differential expression of an γ-aminobutyrate transaminase gene, OsGABA-T, in rice (Oryza sativa) leaves infected with blast fungus. J Plant Res 119, 663–669 (2006). https://doi.org/10.1007/s10265-006-0018-3

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