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Effect of ABA upon anthocyanin synthesis in regenerated torenia shoots

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Abstract

To elucidate the mechanism of anthocyanin synthesis induction concomitant with chlorophyll degradation, we established a system in which anthocyanin synthesis and degradation of chlorophyll in regenerated torenia (Torenia fournieri) shoots was induced on medium containing 7% sucrose. Here, we studied the effect of several plant-growth regulators on anthocyanin synthesis and the degradation of chlorophyll in the torenia shoot regenerating system. Exogenous abscisic acid (ABA) could induce anthocyanin synthesis and chlorophyll senescence in regenerating torenia shoots on the medium containing a low concentration of sucrose (1.5%). We determined the changes in the amount of endogenous ABA in the regenerated shoots during the process of anthocyanin synthesis on the medium containing 7% sucrose. It was revealed that the 7% sucrose treatment elevated endogenous ABA levels before the induction of anthocyanin synthesis and chlorophyll degradation. However, while retransfer to the 1.5% sucrose medium resulted in a gradual decrease in the ABA level and a failure of induction of anthocyanin synthesis, normal shoot regeneration. These results suggest that changes in the amount of endogenous ABA may play an important role in the induction of anthocyanin synthesis and chlorophyll degradation in regenerated torenia shoots.

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Acknowledgments

A part of this work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Koganei, Tokyo, 184-8588, Japan

    Yozo Nagira & Yoshihiro Ozeki

  2. Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Hachioji, Tokyo, Japan

    Keiichi Ikegami & Tomokazu Koshiba

Authors
  1. Yozo Nagira
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  2. Keiichi Ikegami
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  3. Tomokazu Koshiba
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  4. Yoshihiro Ozeki
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Correspondence to Yoshihiro Ozeki.

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Nagira, Y., Ikegami, K., Koshiba, T. et al. Effect of ABA upon anthocyanin synthesis in regenerated torenia shoots. J Plant Res 119, 137–144 (2006). https://doi.org/10.1007/s10265-005-0256-9

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  • DOI: https://doi.org/10.1007/s10265-005-0256-9

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