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Characterization and primer development for amplification of chloroplast microsatellite regions of Fraxinus excelsior

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Abstract

This study reports the characterization of chloroplast DNA (cpDNA) variation of Fraxinus excelsior at five loci and the successful development of primer pairs for the amplification of three of these containing mononucleotide microsatellites. We detected high levels of haplotype variation among provenances of Fraxinus around Europe and within Ireland.

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Acknowledgements

Thanks to Teagasc Irish Agriculture and Food Development Authority and the Walsh Fellowship Programme for funding this project.

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Authors and Affiliations

  1. Department of Botany, University of Dublin, Trinity College, Dublin 2, Ireland

    M. E. Harbourne, S. Waldren & T. R. Hodkinson

  2. Teagasc, Kinsealy Research Centre, Malahide Road, Dublin 17, Ireland

    M. E. Harbourne & G. C. Douglas

Authors
  1. M. E. Harbourne
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  2. G. C. Douglas
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  3. S. Waldren
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  4. T. R. Hodkinson
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Correspondence to M. E. Harbourne.

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Harbourne, M.E., Douglas, G.C., Waldren, S. et al. Characterization and primer development for amplification of chloroplast microsatellite regions of Fraxinus excelsior . J Plant Res 118, 339–341 (2005). https://doi.org/10.1007/s10265-005-0223-5

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  • DOI: https://doi.org/10.1007/s10265-005-0223-5

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