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Analysis of expressed sequence tags in prothallia of Adiantum capillus-veneris

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Abstract

The analysis of expressed sequences from a diverse set of plant species has fueled the increase in understanding of the complex molecular mechanisms underlying plant growth regulation. While representative data sets can be found for the major branches of plant evolution, fern species data are lacking. To further the availability of genetic information in pteridophytes, a normalized cDNA library of Adiantum capillus-veneris was constructed from prothallia grown under white light. A total of 10,420 expressed sequence tags (ESTs) were obtained and clustering of these sequences resulted in 7,100 nonredundant clusters. Of these, 1,608 EST clusters were found to be similar to sequences of known function and 1,092 EST clusters showed similarity to sequences of unknown function. Given the usefulness of Adiantum for developmental studies, the sequence data represented in this report stand to make a significant contribution to the understanding of plant growth regulation, particularly for pteridophytes.

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References

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    Article  CAS  PubMed  Google Scholar 

  • Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408:796–815

    Google Scholar 

  • Asamizu E, Nakamura Y, Sato S, Tabata S (2000) Generation of 7,137 non-redundant expressed sequence tags from a legume, Lotus japonicus. DNA Res 7:127–130

    PubMed  Google Scholar 

  • Cartinhour SW (1997) Public informatics resources for rice and other grasses. Plant Mol Biol 35:241–251

    Article  CAS  PubMed  Google Scholar 

  • Chatterjee A, Roux SJ (2000) Ceratopteris richardii: a productive model for revealing secrets of signaling and development. J Plant Growth Regul 19:284–289

    Article  CAS  PubMed  Google Scholar 

  • Di Laurenzio L, Wysocka-Diller J, Malamy JE, Pysh L, Helariutta Y, Freshour G, Hahn MG, Feldmann KA, Benfey PN (1996) The SCARECROW gene regulates an asymmetric cell division that is essential for generating the radial organization of the Arabidopsis root. Cell 86:423–433

    Article  CAS  PubMed  Google Scholar 

  • Furuya M, Kanno M, Okamoto H, Fukuda S, Wada M (1997) Control of mitosis by phytochrome and a blue-light receptor in fern spores. Plant Physiol 113:677–683

    CAS  PubMed  Google Scholar 

  • Goff SA, Ricke D, Lan TH, Presting G, Wang R, Dunn M, Glazebrook J, Sessions A, Oeller P, Varma H, Hadley D, Hutchison D, Martin C, Katagiri F, Lange BM, Moughamer T, Xia Y, Budworth P, Zhong J, Miguel T, Paszkowski U, Zhang S, Colbert M, Sun W-L, Chen L, Cooper B, Park S, Wood TC, Mao L, Quail P, Wing R, Dean R, Yu Y, Zharkikh A, Shen R, Sahasrabudhe S, Thomas A, Cannings R, Gutin A, Pruss D, Reid J, Tavtigian S, Mitchell J, Eldredge G, Scholl T, Miller RM, Bhatnagar S, Adey N, Rubano T, Tusneem N, Robinson R, Feldhaus J, Macalma T, Oliphant A, Briggs S (2002) A draft sequence of the rice genome (Oryza sativa L. ssp. japonica). Science 296:92–100

    Article  CAS  PubMed  Google Scholar 

  • Hasebe M, Wolf PG, Pryer KM, Ueda K, Ito M, Sano R, Gastony GJ, Yokoyama J, Manhart JR, Murakami N, Crane EH, Haufler CH, Hauk WD (1995) Fern phylogeny based on rbcL nucleotide sequences. Am Fern J 85:134–181

    Google Scholar 

  • Hasebe M, Wen C-K, Kato M, Banks JA (1998) Characterization of MADS homeotic genes in the fern Ceratopteris richardii. Proc Natl Acad Sci USA 95:6222–6227

    Article  CAS  PubMed  Google Scholar 

  • Henschel K, Kofuji R, Hasebe M, Saedler H, Münster T, Theißen G (2002) Two ancient classes of MIKC-type MADS-box genes are present in the moss Physcomitrella patens. Mol Biol Evol 19:801–814

    CAS  PubMed  Google Scholar 

  • Himi S, Sano R, Nishiyama T, Tanahashi T, Kato M, Ueda K, Hasebe M (2001) Evolution of MADS-box gene induction by FLO/LFY genes. J Mol Evol 53:387–393

    Article  CAS  PubMed  Google Scholar 

  • Hiratsuka K, Wu X, Fukuzawa H, Chua N-H (1994) Molecular dissection of GT-1 from Arabidopsis. Plant Cell 6:1805–1813

    Article  CAS  PubMed  Google Scholar 

  • Imaizumi T, Kanegae T, Wada M (2000) Cryptochrome nucleocytoplasmic distribution and gene expression are regulated by light quality in the fern Adiantum capillus-veneris. Plant Cell 12:81–95

    Article  CAS  PubMed  Google Scholar 

  • Jarillo JA, Capel J, Tang RH, Yang H-Q, Alonso JM, Ecker JR, Cashmore AR (2001) An Arabidopsis circadian clock component interacts with both CRY1 and phyB. Nature 410:487–490

    Article  CAS  PubMed  Google Scholar 

  • Kagawa T, Kasahara M, Abe T, Yoshida S, Wada M (2004) Function analysis of phototropin2 using fern mutants deficient in blue light-induced chloroplast avoidance movement. Plant Cell Physiol 45:416–426

    Article  CAS  PubMed  Google Scholar 

  • Kanegae T, Wada M (1998) Isolation and characterization of homologues of plant blue-light photoreceptor (cryptochrome) genes from the fern Adiantum capillus-veneris. Mol Gen Genet 259:345–353

    Article  CAS  PubMed  Google Scholar 

  • Kawai H, Kanegae T, Christensen S, Kiyosue T, Sato Y, Imaizumi T, Kadota A, Wada M (2003) Responses of ferns to red light are mediated by an unconventional photoreceptor. Nature 421:287–290

    Article  CAS  PubMed  Google Scholar 

  • Kawai-Toyooka H, Kuramoto C, Orui K, Motoyama K, Kikuchi K, Kanegae T, Wada M (2004) DNA interference: a simple and efficient gene-silencing system for high-throughput functional analysis in the fern Adiantum. Plant Cell Physiol 45:1648–1657

    Article  CAS  PubMed  Google Scholar 

  • Klein J, Saedler H, Huijser P (1996) A new family of DNA binding proteins includes putative transcriptional regulators of the Antirrhinum majus floral meristem identity gene SQUAMOSA. Mol Gen Genet 250:7–16

    Article  CAS  PubMed  Google Scholar 

  • Klink VP, Wolniak SM (2000) The efficacy of RNAi in the study of the plant cytoskeleton. J Plant Growth Regul 19:371–384

    CAS  PubMed  Google Scholar 

  • Kohchi T, Fujishige K, Ohyama K (1995) Construction of an equalized cDNA library from Arabidopsis thaliana. Plant J 8:771–776

    Article  CAS  PubMed  Google Scholar 

  • Kumar A, Bennetzen JL (1999) Plant retrotransposons. Annu Rev Genet 33:479–532

    Article  CAS  PubMed  Google Scholar 

  • Motchoulski A, Liscum E (1999) Arabidopsis NPH3: a NPH1 photoreceptor-interacting protein essential for phototropism. Science 286:961–964

    Article  CAS  PubMed  Google Scholar 

  • Münster T, Pahnke J, Di Rosa A, Kim JT, Martin W, Saedler H, Theissen G (1997) Floral homeotic genes were recruited from homologous MADS-box genes preexisting in the common ancestor of ferns and seed plants. Proc Natl Acad Sci USA 94:2415–2420

    Article  PubMed  Google Scholar 

  • Nishiyama T, Fujita T, Shin-I T, Seki M, Nishide H, Uchiyama I, Kamiya A, Carninci P, Hayashizaki Y, Shinozaki K, Kohara Y, Hasebe M (2003) Comparative genomics of Physcomitrella patens gametophytic transcriptome and Arabidopsis thaliana: implication for land plant evolution. Proc Natl Acad Sci USA 100:8007–8012

    Article  CAS  PubMed  Google Scholar 

  • Nozue K, Kanegae T, Wada M (1997) A full length Ty3/gypsy-type retrotransposon in the fern Adiantum. J Plant Res 110:495–499

    CAS  Google Scholar 

  • Nozue K, Fukuda S, Kanegae T, Wada M (1998a) Isolation of a second phytochrome cDNA from Adiantum capillus-veneris (accession no. AB016232). Plant Physiol 118:712

    Google Scholar 

  • Nozue K, Kanegae T, Imaizumi T, Fukuda S, Okamoto H, Yeh K-C, Lagarias JC, Wada M (1998b) A phytochrome from the fern Adiantum with features of the putative photoreceptor NPH1. Proc Natl Acad Sci USA 95:15826–15830

    Article  CAS  Google Scholar 

  • Nozue K, Christie JM, Kiyosue T, Briggs WR, Wada M (2000) Isolation and characterization of a fern phototropin (accession no. AB037188). A putative blue-light photoreceptor for phototropism. Plant Physiol 122:1458

    Google Scholar 

  • Okamoto H, Hirano Y, Abe H, Tomizawa K, Furuya M, Wada M (1993) The deduced amino acid sequence of phytochrome from Adiantum includes consensus motifs present in phytochrome B from seed plants. Plant Cell Physiol 34:1329–1334

    CAS  Google Scholar 

  • Putterill J, Robson F, Lee K, Simon R, Coupland G (1995) The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors. Cell 80:847–857

    Article  CAS  PubMed  Google Scholar 

  • Rensing SA, Rombauts S, van de Peer Y, Reski R (2002) Moss transcriptome and beyond. Trends Plant Sci 7:535–538

    Article  CAS  PubMed  Google Scholar 

  • Schaefer DG, Zrÿd J-P (1997) Efficient gene targeting in the moss Physcomitrella patens. Plant J 11:1195–1206

    Article  CAS  PubMed  Google Scholar 

  • Staut SC, Clark GB, Archer-Evans SA, Roux SJ (2003) Rapid and efficient suppression of gene expression in a single-cell model system, Ceratopteris richardii. Plant Physiol 131:1165–1168

    Article  PubMed  Google Scholar 

  • Unte US, Sorensen A-M, Pesaresi P, Gandikota M, Leister D, Saedler H, Huijser P (2003) SPL8, an SBP-box gene that affects pollen sac development in Arabidopsis. Plant Cell 15:1009–1019

    Article  CAS  PubMed  Google Scholar 

  • Wada M, Furuya M (1978) Effects of narrow-beam irradiations with blue and far-red light on the timing of cell division in Adiantum gametophytes. Planta 138:85–90

    Article  Google Scholar 

  • Wada M, Kadota A (1989) Photomorphogenesis in lower green plants. Annu Rev Plant Physiol Plant Mol Biol 40:169–191

    Article  Google Scholar 

  • Wada M, Sugai M (1994) Photobiology of ferns. In: Kendrick RE, Kronenberg GHM (eds) Photomorphogenesis in plants, 2nd edn. Kluwer Academic, Dordrecht, pp 783–802

    Google Scholar 

  • Wada M, Kagawa T, Sato Y (2003) Chloroplast movement. Annu Rev Plant Biol 54:455–468

    Article  CAS  PubMed  Google Scholar 

  • Wolf PG, Rowe CA, Sinclair RB, Hasebe M (2003) Complete nucleotide sequence of the chloroplast genome from a leptosporangiate fern, Adiantum capillus-veneris L. DNA Res 10:59–65

    CAS  PubMed  Google Scholar 

  • Yamamoto YY, Matsui M, Ang L-H, Deng X-W (1998) Role of a COP1 interactive protein in mediating light-regulated gene expression in Arabidopsis. Plant Cell 10:1083–1094

    Article  CAS  PubMed  Google Scholar 

  • Yu J, Hu S, Wang J, Wong GK, Li S, Liu B, Deng Y, Dai L, Zhou Y, Zhang X, Cao M, Liu J, Sun J, Tang J, Chen Y, Huang X, Lin W, Ye C, Tong W, Cong L, Geng J, Han Y, Li L, Li W, Hu G, Huang X, Li W, Li J, Liu Z, Li L, Liu J, Qi Q, Liu J, Li L, Li T, Wang X, Lu H, Wu T, Zhu M, Ni P, Han H, Dong W, Ren X, Feng X, Cui P, Li X, Wang H, Xu X, Zhai W, Xu Z, Zhang J, He S, Zhang J, Xu J, Zhang K, Zheng X, Dong J, Zeng W, Tao L, Ye J, Tan J, Ren X, Chen X, He J, Liu D, Tian W, Tian C, Xia H, Bao Q, Li G, Gao H, Cao T, Wang J, Zhao W, Li P, Chen W, Wang X, Zhang Y, Hu J, Wang J, Liu S, Yang J, Zhang G, Xiong Y, Li Z, Mao L, Zhou C, Zhu Z, Chen R, Hao B, Zheng W, Chen S, Guo W, Li G, Liu S, Tao M, Wang J, Zhu L, Yuan L, Yang H (2002) A draft sequence of the rice genome (Oryza sativa L. ssp. indica). Science 296:79–92

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We are grateful to Dr. T. Demura at RIKEN Plant Science Center for construction of the cDNA library and to E. Sugiyama and T. Yasuki for collecting spores of Adiantum capillus-veneris. We thank Dr. Steen Christensen for critical reading of the manuscript. This work was partly supported by BRAIN (Program for Promotion of Basic Research Activities for Innovative Biosciences) and Grant-in-Aid for Scientific Research (on Priority Areas, no. 13139203, and A, no. 13304061) from the Ministry of Education, Sports, Science and Technology (MEXT) of Japan to MW.

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Authors and Affiliations

  1. Department of Life Science, University of Hyogo, 2167 Shosha, Himeji, Hyogo, 671-2201, Japan

    Daisuke Yamauchi

  2. Department of Regulatory Biology, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, 444-8585, Japan

    Daisuke Yamauchi & Masamitsu Wada

  3. Department of Biological Sciences, Tokyo Metropolitan University, 1-1 Minamiosawa, Hachioji, Tokyo, 192-0397, Japan

    Keita Sutoh, Hiromi Kanegae & Masamitsu Wada

  4. Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan

    Tatsuya Horiguchi, Ken Matsuoka & Hiroo Fukuda

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  1. Daisuke Yamauchi
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Correspondence to Daisuke Yamauchi.

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Yamauchi, D., Sutoh, K., Kanegae, H. et al. Analysis of expressed sequence tags in prothallia of Adiantum capillus-veneris. J Plant Res 118, 223–227 (2005). https://doi.org/10.1007/s10265-005-0209-3

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  • DOI: https://doi.org/10.1007/s10265-005-0209-3

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