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Hypocholesterolemia is associated negatively with hemolysate lipid peroxidation in sickle cell anemia patients

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An Erratum to this article was published on 04 September 2011

Abstract

The oxidative stress levels in plasma and hemolysate and cholesterol levels in plasma of sickle cell anemia patients, carriers and controls were evaluated. A total of 40 cases—17 patients, 13 carriers and 10 controls—were involved in the study. Plasma and hemolysate malondialdehyde (MDA) were detected via thiobarbituric acid reaction with a fluorimetric detector by high-performance liquid chromatography system. Plasma cholesterol was determined by enzymatic colorimetric method. Mean MDA levels of SCA patients were higher than those of the carriers’ and healthy children’s both in plasma and in hemolysate (P < 0.005). The mean plasma and hemolysate MDA levels were 25.3 ± 1.6 nmol/l and 86.7 ± 19.3 nmol/l in patients, 19.1 ± 0.8 nmol/l and 54.1 ± 10.8 nmol/l in carriers and 19.6 ± 0.8 nmol/l and 56.8 ± 9.3 nmol/l in healthy children. Mean plasma total cholesterol levels were 92.1 ± 19.1 mg/dl in patients, 116.2 ± 23.3 mg/dl in carriers and 126.6 ± 16.4 mg/dl in controls (P < 0.005). There was a significant negative correlation of −0.520 between hemolysate MDA and plasma cholesterol levels in patients (P < 0.05). The degree of correlation increased up to −0.782 (P = 0.008) in the patients with HbSS phenotype. This negative correlation between MDA and cholesterol may imply a potential association between oxidative stress and hypocholesterolemia in sickle cell anemia.

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References

  1. Rother R, Bell L, Hillmen P, Gladwin M (2005) The clinical sequelae of intravascular hemolysis and extracellular plasma hemoglobin: a novel mechanism of human disease. JAMA 293:1653–1662

    Article  PubMed  CAS  Google Scholar 

  2. Hebbel RP (1985) Auto-oxidation and a membrane-associated ‘Fenton reagent’: a possible explanation for development of membrane lesions in sickle erythrocytes. Clin Haematol 14:129–140

    PubMed  CAS  Google Scholar 

  3. Shalev O, Hebbel RP (1996) Catalysis of soluble hemoglobin oxidation by free iron on sickle red cell membranes. Blood 87:3948–3952

    PubMed  CAS  Google Scholar 

  4. Buchanan GR, Holtkamp CA (1981) Platelet aggregation, malondialdehyde generation and production time in children with sickle cell anaemia. Thromb Haemost 46:690–693

    PubMed  CAS  Google Scholar 

  5. Walter PB, Fung EB, Killilea DW, Jiang Q, Hudes M, Madden J, Porter J, Evans P, Vichinsky E, Harmatz P (2006) Oxidative stress and inflammation in iron-overloaded patients with beta-thalassaemia or sickle cell disease. Br J Haematol 135:254–263

    Article  PubMed  CAS  Google Scholar 

  6. Jain SK (1984) The accumulation of malonyldialdehyde, a product of fatty acid peroxidation, can disturb aminophospholipid organization in the membrane bilayer of human erythrocytes. J Biol Chem 259:3391–3394

    PubMed  CAS  Google Scholar 

  7. Bereza UL, Brewer GJ, Hill GM (1985) Effect of dietary cholesterol on erythrocyte peroxidant stress in vitro and in vivo. Biochim Biophys Acta 835:434–440

    PubMed  CAS  Google Scholar 

  8. Buchowski MS, Swift LL, Akohoue SA, Shankar SM, Flakoll PJ, Abumrad N (2007) Defects in postabsorptive plasma homeostasis of fatty acids in sickle cell disease. JPEN J Parenter Enteral Nutr 31:263–268

    Article  PubMed  CAS  Google Scholar 

  9. Westerman MP, Pierce LE, Jensen WN (1964) Erythrocyte and plasma lipids in sickle cell anemia. Blood 23:200–205

    PubMed  CAS  Google Scholar 

  10. VanderJagt DJ, Shores J, Okorodudu A, Okolo SN, Glew RH (2002) Hypocholesterolemia in Nigerian children with sickle cell disease. J Trop Pediatr 48:156–161

    Article  PubMed  Google Scholar 

  11. Rahimi Z, Merat A, Haghshenass M, Madani H, Rezaei M, Nagel RL (2006) Plasma lipids in Iranians with sickle cell disease: hypocholesterolemia in sickle cell anemia and increase of HDL-cholesterol in sickle cell trait. Clin Chim Acta 365:217–220

    Article  PubMed  CAS  Google Scholar 

  12. Shores J, Peterson J, VanderJagt D, Glew RH (2003) Reduced cholesterol levels in African-American adults with sickle cell disease. J Natl Med Assoc 95:813–817

    PubMed  Google Scholar 

  13. Draper HH, Squires EJ, Mahmoodi H, Wu J, Agarwal S, Hadley M (1993) A comparative evaluation of thiobarbituric acid methods for the determination of malondialdehyde in biological materials. Free Radic Biol Med 15:353–363

    Article  PubMed  CAS  Google Scholar 

  14. Templar J, Kon SP, Milligan TP, Newman DJ, Raftery MJ (1999) Increased plasma malondialdehyde levels in glomerular disease as determined by a fully validated HPLC method. Nephrol Dial Transplant 14:946–951

    Article  PubMed  CAS  Google Scholar 

  15. Chiu D, Vichinsky E, Yee M, Kleman K, Lubin B (1982) Peroxidation, vitamin E, and sickle-cell anemia. Ann N Y Acad Sci 393:323–335

    Article  PubMed  CAS  Google Scholar 

  16. Zorca S, Freeman L, Hildesheim M, Allen D, Remaley AT, Taylor JG, Kato GJ (2010) Lipid levels in sickle-cell disease associated with haemolytic severity, vascular dysfunction and pulmonary hypertension. Br J Haematol 149:436–445

    Google Scholar 

  17. Yokoyama M, Suto Y, Sato H, Arai K, Waga S, Kitazawa J, Maruyama H, Ito E (2000) Low serum lipids suggest severe bone marrow failure in children with aplastic anemia. Pediatr Int 42:613–619

    Article  PubMed  CAS  Google Scholar 

  18. Johnsson R, Saris NE (1981) Plasma and erythrocyte lipids in hereditary spherocytosis. Clin Chim Acta 114:263–268

    Article  PubMed  CAS  Google Scholar 

  19. Papanastasiou DA, Siorokou T, Haliotis FA (1996) beta-Thalassaemia and factors affecting the metabolism of lipids and lipoproteins. Haematologia (Budap) 27:143–153

    CAS  Google Scholar 

  20. Shalev H, Kapelushnik J, Moser A, Knobler H, Tamary H (2007) Hypocholesterolemia in chronic anemias with increased erythropoietic activity. Am J Hematol 82:199–202

    Article  PubMed  CAS  Google Scholar 

  21. Sasaki J, Waterman MR, Buchanan GR, Cottam GL (1983) Plasma and erythrocyte lipids in sickle cell anaemia. Clin Lab Haematol 5:35–44

    Article  PubMed  CAS  Google Scholar 

  22. Ngogang J, Mouray H, Lebreton de Vonne T, Raisonnier A (1989) Erythrocyte and plasma cholesterol exchange in sickle cell anemia. Clin Chim Acta 179:295–304

    Article  PubMed  CAS  Google Scholar 

  23. Fasola F, Adedapo K, Anetor J, Kuti M (2007) Total antioxidants status and some hematological values in sickle cell disease patients in steady state. J Natl Med Assoc 99:891–894

    PubMed  Google Scholar 

  24. Seixas MO, Rocha LC, Carvalho MB, Menezes JF, Lyra IM, Nascimento VM, Couto RD, Atta AM, Reis MG, Goncalves MS (2010) Levels of high-density lipoprotein cholesterol (HDL-C) among children with steady-state sickle cell disease. Lipids Health Dis 9:91

    Article  PubMed  Google Scholar 

  25. Oztas Y, Unal S, Ozgunes N (2009) Plasma protein oxidation and triglyceride levels in sickle cell anemia. Blood (ASH Annual Meeting Abstracts) 114:2578

    Google Scholar 

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Acknowledgments

This work is supported by part of a grant from Scientific Research Unit of Hacettepe University (07D09101006).

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Authors and Affiliations

  1. Faculty of Medicine, Department of Clinical Biochemistry, Hacettepe University, Rektorluk Binası, Kat: 3 Sihhiye, Ankara, Turkey

    Oztas Er Yesim & Ozgunes Nuriman

  2. Faculty of Pharmacy, Department of Toxicology, Hacettepe University, Ankara, Turkey

    Sabuncuoglu Suna & Ozgunes Hilal

  3. Faculty of Medicine, Department of Pediatrics, Hematology Unit, Mersin University, Mersin, Turkey

    Unal Selma

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  1. Oztas Er Yesim
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  2. Sabuncuoglu Suna
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  3. Unal Selma
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  4. Ozgunes Hilal
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  5. Ozgunes Nuriman
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Correspondence to Ozgunes Nuriman.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s10238-011-0156-3

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Yesim, O.E., Suna, S., Selma, U. et al. Hypocholesterolemia is associated negatively with hemolysate lipid peroxidation in sickle cell anemia patients. Clin Exp Med 11, 195–198 (2011). https://doi.org/10.1007/s10238-010-0124-3

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  • DOI: https://doi.org/10.1007/s10238-010-0124-3

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