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The influence of mechanical stimulation on osteoclast localization in the mouse maxilla: bone histomorphometry and finite element analysis

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Abstract

The mechanism of traumatic bone resorption in the denture-bearing bone has not yet been established with regard to the osteoclastic activity in relation to the mechanical stimulus. The purpose of this study was to clarify whether osteoclast appearance in maxilla depends on the strain intensity, using the murine loading model. The maxillary palate of thirteen-week-old male C57BL/6 mice was subjected to continuous pressure of 2 kPa (low stimulation, n = 4) or 7 kPa (high stimulation, n = 4) for 30 min/day for 7 consecutive days, and the mice were sacrificed after the last loading. The control group underwent the same protocol without load (n = 4). An animal-specific finite element model was constructed based on morphology and characteristics obtained from the micro-CT data and used to calculate the strain intensity of the bone. The bone histomorphometric technique revealed significant reduction of cortical bone volume and significant increase of bone resorption parameters such as osteoclast number in the bone tissue under the loading contact in comparison to the control (p < 0.05). The osteoclasts were observed in the subsurface region adjacent to the loading contact and the peripheral region of the marrow space in the intracortical region of the cortical bone in the mouse maxilla in both stimulation groups. An average of more than 90 % of the osteoclasts was observed in the areas with strain intensity higher than 85.0μ strain for the high stimulation group. The result suggests that the osteoclastic resorption is location-dependent and is also sensitive to the local strain intensity.

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Authors and Affiliations

  1. Department of Removable Partial Prosthodontics, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo, Tokyo, 113-8549, Japan

    K. Fujiki, Y. Igarashi & N. Wakabayashi

  2. Section of Pharmacology, Department of Bio-Matrix, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan

    K. Aoki & K. Ohya

  3. Institute of Solid Mechanics, Mechatronics and Biomechanics, Brno University of Technology, Brno, Czech Republic

    P. Marcián & L. Borák

  4. Section of Periodontology, Department of Preventive Dentistry, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan

    M. Hudieb

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  1. K. Fujiki
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  2. K. Aoki
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  3. P. Marcián
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  4. L. Borák
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  5. M. Hudieb
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  6. K. Ohya
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  7. Y. Igarashi
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  8. N. Wakabayashi
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Correspondence to N. Wakabayashi.

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Fujiki, K., Aoki, K., Marcián, P. et al. The influence of mechanical stimulation on osteoclast localization in the mouse maxilla: bone histomorphometry and finite element analysis. Biomech Model Mechanobiol 12, 325–333 (2013). https://doi.org/10.1007/s10237-012-0401-z

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  • DOI: https://doi.org/10.1007/s10237-012-0401-z

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