Abstract
Ex vivo culture of arteries and veins is an established tool for investigating mechanically induced remodeling. Porcine saphenous veins (PSV) cultured ex vivo with a venous mechanical environment, serum-supplemented cell-culture medium and standard cell-culture conditions (5% CO2 and 95% balance air ~140 mmHg pO2) develop intimal hyperplasia (IH), increased cellular proliferation, decreased compliance and exhibit inward eutrophic remodeling thereby suggesting that nonmechanical factors stimulate some changes observed ex vivo. Herein we explore the contribution of exposure to greater than venous pO2 and serum to these changes in cultured veins. Removing serum from culture medium did not inhibit development of IH, but did reduce cellular proliferation and inward eutrophic remodeling. In contrast, veins perfused using reduced pO2 (75 mmHg) showed reduced IH. Among the statically cultured vessels, veins cultured at arterial pO2 (95 mmHg) and above showed IH as well as increase in proliferation and vessel weight compared to fresh veins; veins cultured at venous pO2 did not. Taken together, these data suggest that exposure of SV to arterial pO2 stimulates IH and cellular proliferation independent of changes in the mechanical environment, which might provide insight into the etiology of IH in SV used as arterial grafts.
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This work was supported by AHA (grants 0555538U and 0655323B) to KJG.
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Open Access This is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
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Joddar, B., Shaffer, R.J.G., Reen, R.K. et al. Arterial pO2 stimulates intimal hyperplasia and serum stimulates inward eutrophic remodeling in porcine saphenous veins cultured ex vivo. Biomech Model Mechanobiol 10, 161–175 (2011). https://doi.org/10.1007/s10237-010-0224-8
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DOI: https://doi.org/10.1007/s10237-010-0224-8