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Leukocytapheresis (LCAP) decreases the level of platelet-derived microparticles (MPs) and increases the level of granulocytes-derived MPs: a possible connection with the effect of LCAP on rheumatoid arthritis

  • Original Article
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Modern Rheumatology

Abstract

Microparticles (MPs) are believed to play an important role in inflammatory diseases such as rheumatoid arthritis (RA). Leukocytapheresis (LCAP) is one of the options available for the treatment of RA. We analyzed the levels of MPs in RA, by flow cytometry, especially in relation to the effect of LCAP. Twenty female patients with RA were recruited into this study. Six of the 20 patients with RA further received LCAP. Plasma levels of platelet-derived MPs were high in patients with RA and are correlated with disease activity. LCAP significantly improved RA in all six patients. The numbers of platelet-derived MPs significantly decreased after the first session of LCAP, which was probably due to direct removal by LCAP. Mean numbers of platelet-derived MPs after four sessions of LCAP markedly decreased. The numbers of granulocyte-derived MPs, which are suggested to have an anti-inflammatory effect, were markedly increased after the first session of LCAP. These data suggest that removal of platelet-derived MPs and increase of granulocyte-derived MPs are novel mechanisms of LCAP as effective treatment in RA.

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Acknowledgments

We thank Drs. Y. Aratake, Y. Ogata, I. Kobayashi, and S. Shakano (University of Miyazaki) for their technical assistance.

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Authors and Affiliations

  1. Department of Rheumatology, Infectious Diseases, and Laboratory Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan

    Kunihiko Umekita, Shiro Ueno, Ichiro Takajo, Yasufumi Kai, Yasuhiro Nagatomo & Akihiko Okayama

  2. Institute of Rheumatology, Zenjinkai Shimin-No-Mori Hospital, Miyazaki, Japan

    Toshihiko Hidaka

  3. Department of Anatomy, Ultrastructural Cell Biology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan

    Akira Sawaguchi & Tatsuo Suganuma

Authors
  1. Kunihiko Umekita
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  2. Toshihiko Hidaka
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  3. Shiro Ueno
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  4. Ichiro Takajo
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  9. Akihiko Okayama
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Correspondence to Akihiko Okayama.

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Umekita, K., Hidaka, T., Ueno, S. et al. Leukocytapheresis (LCAP) decreases the level of platelet-derived microparticles (MPs) and increases the level of granulocytes-derived MPs: a possible connection with the effect of LCAP on rheumatoid arthritis. Mod Rheumatol 19, 265–272 (2009). https://doi.org/10.1007/s10165-009-0164-2

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  • DOI: https://doi.org/10.1007/s10165-009-0164-2

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