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Histochemical and immunoelectron microscopic analysis of ganglioside GM3 in human kidney

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Abstract

Background

Gangliosides are amphipathic lipids ubiquitously expressed in all vertebrate cells. They have been reported to play pivotal roles in cell morphology, cell adhesion, signal transduction, and modulation of immune reaction. Although human kidney contains various kinds of ganglioside, their physiological and pathophysiological roles have not been elucidated yet. As ganglioside GM3 is the most abundant ganglioside in human kidney, we tried to reveal the distribution of GM3 using histological analysis.

Methods

Macroscopically normal parts of operatively resected kidney from renal cell carcinoma patients were used for analyses. Immunohistochemical and immunoelectron microscopic analyses were performed with anti-GM3 antibody.

Results

Immunohistochemical analyses showed that GM3 was observed in glomeruli and renal proximal tubules. Immunoelectron microscopy demonstrated that GM3 was localized on the foot process of podocyte and also in Golgi region of renal proximal tubule cells.

Conclusions

Ganglioside GM3 might take a part of the negative electric charge on the surface of podocyte and its multiple physiological actions may play pivotal roles for maintaining glomerular function.

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Acknowledgments

We thank Ms. Sachie Matubara (Laboratory for Electron microscopy, Kyorin University School of Medicine) for electron microscopic analyses. We also thank Dr. Takao Taki (Tokushima Institute, Otsuka Pharmaceutial Co., Ltd), for providing control samples of ganglioside, technical advice and helpful discussion.

Conflict of interest

Potential financial conflict of interest.

Honoraria: Yoshiharu Tsubakihara (Chugai Pharm Co., Ltd, Kyowa Hakko, Kirin Co.,Ltd, Mitsubishi Tanabe Co., Ltd, Bayer Co., Ltd,), Research funding: Yoshiharu Tsubakihara (Chugai Pharm Co., Ltd, Baxter Japan Ltd, Otsuka Pharm Co., Ltd, Bayer Co., Ltd,), Endowed departments by commercial entities: Yoshiharu Tsubakihara (Chugai Pharm Co., Ltd, Baxter Japan Ltd,). The other authors have declared that no conflict of interest exists.

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Authors and Affiliations

  1. Department of Nephrology, NTT West Japan Osaka Hospital, Osaka, Japan

    Tetsuya Kaneko

  2. Department of Comprehensive Kidney Disease Research, Osaka University Graduate School of Medicine, Osaka, Japan

    Yoshiharu Tsubakihara

  3. The Department of Pathology, Osaka General Medical Center, Osaka, Japan

    Hiroaki Fushimi

  4. The Department of Urology, Osaka General Medical Center, Osaka, Japan

    Seiji Yamaguchi

  5. Department of Geriatric Medicine and Nephrology, Osaka University Graduate School of Medicine, Osaka, Japan

    Yoshitsugu Takabatake, Hiromi Rakugi & Yoshitaka Isaka

  6. Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, Japan

    Hayato Kawakami

Authors
  1. Tetsuya Kaneko
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  2. Yoshiharu Tsubakihara
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Correspondence to Tetsuya Kaneko.

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Kaneko, T., Tsubakihara, Y., Fushimi, H. et al. Histochemical and immunoelectron microscopic analysis of ganglioside GM3 in human kidney. Clin Exp Nephrol 19, 403–410 (2015). https://doi.org/10.1007/s10157-014-1003-0

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  • DOI: https://doi.org/10.1007/s10157-014-1003-0

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