Abstract
Epithelial–mesenchymal transition (EMT) has been well investigated using cultured kidney epithelial cell lines and the mouse obstructive nephropathy model. The role of EMT in the genesis or progression of human renal fibrosis remains controversial. We report a case of obstructive nephropathy that occurred in a pregnant woman. Renal tissue obtained by percutaneous renal biopsy after the delivery showed marked interstitial fibrosis and obstructive tubuli. Immunofluorescence images clearly demonstrated S100A4-positive tubular epithelial cells in these specimens. With regard to human obstructive nephropathy, this is the first report which proves that tubular epithelial cells become positive for the fibroblast-specific marker. EMT is probably a common event not only in mouse but also in human.
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Neilson EG. Setting a trap for tissue fibrosis. Nat Med. 2005;11:373–4.
Strutz F, Okada H, Lo CW, Danoff T, Carone RL, Tomaszewski JE, et al. Identification and characterization of a fibroblast marker: FSP1. J Cell Biol. 1995;130:393–405.
Ford HL, Silver DL, Kachar B, Sellers JR, Zain SB. Effect of Mts1 on the structure and activity of nonmuscle myosin II. Biochemistry. 1997;36:16321–7.
Grigorian M, Andresen S, Tulchinsky E, Kriajevska M, Carlberg C, Kruse C, et al. Tumor suppressor p53 protein is a new target for the metastasis-associated Mts1/S100A4 protein: functional consequences of their interaction. J Biol Chem. 2001;276:22699–708.
Kriajevska M, Tarabykina S, Bronstein I, Maitland N, Lomonosov M, Hansen K, et al. Metastasis-associated Mts1 (S100A4) protein modulates protein kinase C phosphorylation of the heavy chain of nonmuscle myosin. J Biol Chem. 1998;273:9852–6.
Inoue T, Plieth D, Venkov CD, Xu C, Neilson EG. Antibodies against macrophages that overlap in specificity with fibroblasts. Kidney Int. 2005;67:2488–93.
Bhowmick NA, Chytil A, Plieth D, Gorska AE, Dumont N, Shappell S, et al. TGF-beta signaling in fibroblasts modulates the oncogenic potential of adjacent epithelia. Science. 2004;303:848–51.
Rasmussen PE, Nielsen FR. Hydronephrosis during pregnancy: a literature survey. Eur J Obstet Gynecol Reprod Biol. 1988;27:249–59.
Brandes JC, Fritsche C. Obstructive acute renal failure by a gravid uterus: a case report and review. Am J Kidney Dis. 1991;18:398–401.
Jena M, Mitch WE. Rapidly reversible acute renal failure from ureteral obstruction in pregnancy. Am J Kidney Dis. 1996;28:457–60.
Chevalier RL. Obstructive nephropathy: towards biomarker discovery and gene therapy. Nat Clin Pract Nephrol. 2006;2:157–68.
Kaneto H, Ohtani H, Fukuzaki A, Ishidoya S, Takeda A, Ogata Y, et al. Increased expression of TGF-beta1 but not of its receptors contributes to human obstructive nephropathy. Kidney Int. 1999;56:2137–46.
Nishitani Y, Iwano M, Yamaguchi Y, Harada K, Nakatani K, Akai Y, et al. Fibroblast-specific protein 1 is a specific prognostic marker for renal survival in patients with IgAN. Kidney Int. 2005;68:1078–85.
Rossini M, Cheunsuchon B, Donnert E, Ma LJ, Thomas JW, Neilson EG, et al. Immunolocalization of fibroblast growth factor-1 (FGF-1), its receptor (FGFR-1), and fibroblast-specific protein-1 (FSP-1) in inflammatory renal disease. Kidney Int. 2005;68:2621–8.
Gibbs FE, Barraclough R, Platt-Higgins A, Rudland PS, Wilkinson MC, Parry EW. Immunocytochemical distribution of the calcium-binding protein p9Ka in normal rat tissues: variation in the cellular location in different tissues. J Histochem Cytochem. 1995;43:169–80.
Djamali A, Vidyasagar A, Adulla M, Hullett D, Reese S. Nox-2 is a modulator of fibrogenesis in kidney allografts. Am J Transplant. 2009;9:74–82.
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Inoue, T., Okada, H., Takenaka, T. et al. A case report suggesting the occurrence of epithelial–mesenchymal transition in obstructive nephropathy. Clin Exp Nephrol 13, 385–388 (2009). https://doi.org/10.1007/s10157-009-0168-4
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DOI: https://doi.org/10.1007/s10157-009-0168-4