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Molecular characterization of carbapenem-non-susceptible Acinetobacter spp. in Japan: predominance of multidrug-resistant Acinetobacter baumannii clonal complex 92 and IMP-type metallo-β-lactamase-producing non-baumannii Acinetobacter species

  • Original Article
  • Published:
Journal of Infection and Chemotherapy

Abstract

We conducted an epidemiological study concerning carbapenem-non-susceptible clinical isolates of Acinetobacter spp. in Japan by molecular procedures including carbapenemase gene identification and amplified ribosomal DNA restriction analysis. Among 598 clinically isolated Acinetobacter spp. in 2007, 27 (4.5%) were non-susceptible to carbapenems. Most carbapenem-non-susceptible Acinetobacter baumannii (13/14) belonged to clonal complex (CC) 92, harbored bla OXA-51-like genes, including novel bla OXA-206, downstream of ISAba1, and were recovered mainly from the Kanto region. Carbapenem-non-susceptible A. baumannii CC92 isolates were further divided by pulsed-field gel electrophoresis into two groups, one of which was characterized by the presence of bla OXA-23. One A. baumannii CC276 isolate carried bla IMP-1 and bla OXA-58. Almost all non-baumannii Acinetobacter isolates (12/13), including Acinetobacter pittii (formerly Acinetobacter genomic species 3) and Acinetobacter nosocomialis (formerly Acinetobacter genomic species 13TU), produced IMP-type metallo-β-lactamases, and were recovered from various regions in Japan. This is the first report describing the nationwide molecular epidemiology of carbapenem-non-susceptible Acinetobacter spp. with genomic species-level identification in Japan.

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Acknowledgments

The authors thank the technical staff and directors in the 72 hospitals as the Levofloxacin Surveillance Group. We express our deep appreciation to David M. Livermore, Yohei Doi, and Tse Hsien Koh for their critical comments and careful reviewing of the manuscript. This study was supported by the Ministry of Health, Labor, and Welfare of Japan (No. H21-Shinkou-Ippan-008 to K.Y.), and by a grant-in-aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 22591113 to Y.I.). This work was also supported by a grant from Daiichi Sankyo Co., Ltd. to collect Acinetobacter spp. isolates (to K.Y.).

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Authors and Affiliations

  1. Department of Microbiology and Infectious Diseases, Toho University School of Medicine, Omori-nishi, Ota-ku, Tokyo, 1438540, Japan

    Yuichi Kouyama, Sohei Harada, Yoshikazu Ishii, Tomoo Saga, Ayumi Yoshizumi, Kazuhiro Tateda & Keizo Yamaguchi

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  1. Yuichi Kouyama
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  2. Sohei Harada
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  3. Yoshikazu Ishii
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  4. Tomoo Saga
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  5. Ayumi Yoshizumi
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  6. Kazuhiro Tateda
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  7. Keizo Yamaguchi
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Correspondence to Yoshikazu Ishii.

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Kouyama, Y., Harada, S., Ishii, Y. et al. Molecular characterization of carbapenem-non-susceptible Acinetobacter spp. in Japan: predominance of multidrug-resistant Acinetobacter baumannii clonal complex 92 and IMP-type metallo-β-lactamase-producing non-baumannii Acinetobacter species. J Infect Chemother 18, 522–528 (2012). https://doi.org/10.1007/s10156-012-0374-y

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  • DOI: https://doi.org/10.1007/s10156-012-0374-y

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