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RNA-Seq revealed that infection with white tip nematodes could downregulate rice photosynthetic genes

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Abstract

White-tip nematode, Aphelenchoides besseyi is a kind of widely distributed migratory parasitic nematode that can infect plant shoots. Transcriptome sequencing of plant parasitic nematodes and their host plants is helpful for understanding their interaction relationship. This study first reported expression patterns of defense-related genes in rice, and rice transcriptomes at different periods after infection with A. besseyi. The result showed that the defense response pathways of rice changed obviously in the early stage of A. besseyi infection, including upregulated salicylic acid and jasmonate pathways and a downregulated ethylene pathway. Transcriptome analysis results suggested that A. besseyi infection was associated with the downregulation of multiple genes related to photosynthesis with possible suppression of the photosynthetic activity. It suggested that the photosynthesis system of rice could be suppressed by infections of migratory nematodes, including A. besseyi and Hirschmanniella oryzae, but was stimulated by that of a sedentary nematode, Meloidogyne graminicola, by comparing our study with the reported transcriptome. OS09G0417800 (OsWRKY62) might play an important role in the interaction of migratory nematodes and rice. It also indicated that the infection strategy of both A. besseyi and the reported migratory nematode H. oryzae was similar to that of the fungal pathogen Magnaporthe grisea. These results provided an interesting starting point to elucidate the mechanism of the interaction between rice and A. besseyi, as well as the host and migratory plant nematodes.

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Availability of data and materials

All data generated or analyzed during this study are included in this published article and its supplementary information files. Sequences were deposited in SRA (BioProject Accession: PRJNA541104).

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Funding

This work was funded by National Natural Science Foundation of China (No.31871939 and 31371920) and Doctoral innovative talents (domestic training) Cultivation Project of South China Agricultural University in 2019 (No. CX2019N048).

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Authors and Affiliations

  1. Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Department of Plant Pathology/Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou, People’s Republic of China

    Hong-Le Wang, Si-Hua Yang, Mei Lv, Shan-Wen Ding, Jun-Yi Li, Chun-Ling Xu & Hui Xie

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  1. Hong-Le Wang
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  2. Si-Hua Yang
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  7. Hui Xie
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Contributions

HX and HLW designed the experiments; HLW, SHY, ML, SWD, and JYL performed the experiments; HLW, SWD, JYL, and CLX analyzed the data; HLW, HX, and CLX wrote the manuscript. All authors read and approved the final version of the manuscript.

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Correspondence to Hui Xie.

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The authors declare that they have no conflict of interest.

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Animals were treated in strict accordance with the Animal Ethics Procedures and Guidelines of the People’s Republic of China. All animal procedures were approved by the Animal Ethics Committee of the South China Agricultural University.

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Wang, HL., Yang, SH., Lv, M. et al. RNA-Seq revealed that infection with white tip nematodes could downregulate rice photosynthetic genes. Funct Integr Genomics 20, 367–381 (2020). https://doi.org/10.1007/s10142-019-00717-9

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