Abstract
Compositional studies comparing transgenic with non-transgenic counterpart plants are almost universally required by governmental regulatory bodies. In the present study, two T2 transgenic cotton lines containing chitinase (Line 11/57) and Bt lines (Line 61) were compared with non-transgenic counterpart. To do this, biochemical characteristics of leaves and seeds, including amino acids, fatty acids, carbohydrates, anions, and cations contents of the studied lines were analyzed using GC/MS, high-performance liquid chromatography (HPLC), and ion chromatography (IC) analyzers, respectively. polymerase chain reaction (PCR) and Western blot analyses confirmed the presence and expression of Chi and Bt genes in the studied transgenic lines. Although, compositional analysis of leaves contents confirmed no significant differences between transgenic and non-transgenic counterpart lines, but it was shown that glucose content of chitinase lines, fructose content of transgenic lines (Bt and chitinase) and asparagine and glutamine of chitinase lines were significantly higher than the non-transgenic counterpart plants. Both the transgenic lines (Bt and chitinase) showed significant decrease in the amounts of sodium in comparison to the non-transgenic counterpart plants. The experiments on the seeds showed that histidine, isoleucine, leucine, and phenylalanine contents of all transgenic and non-transgenic lines were the same, whereas other amino acids were significantly increased in the transgenic lines. Surprisingly, it was observed that the concentrations of stearic acid, myristic acid, oleic acid, and linoleic acid in the chitinase line were significantly different than those of non-transgenic counterpart plants, but these components were the same in both Bt line and its non-transgenic counterpart. It seems that more changes observed in the seed contents than leaves is via this point that seeds are known as metabolites storage organs, so they show greater changes in the metabolites contents comparing to the leaves.
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Acknowledgments
This work was supported by a grant received from the Agricultural Research and Education Organization of Iran (AREO). We wish to thank all our colleagues in the Department of Plant Tissue Culture and Genetic engineering of Agriculture Biotechnology Research Institute of Iran(ABRII) for their support and technical assistance.
The authors would also like to extend their appreciation to Dr. Motahare Mohsenpoor and Hossein Hadavand for molecular and biochemical analysis and Assoc. Prof. Salehi for his critical review of the manuscript in terms of its academic English writing.
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Modirroosta, B.H., Tohidfar, M., Saba, J. et al. The substantive equivalence of transgenic (Bt and Chi) and non-transgenic cotton based on metabolite profiles. Funct Integr Genomics 14, 237–244 (2014). https://doi.org/10.1007/s10142-013-0355-y
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DOI: https://doi.org/10.1007/s10142-013-0355-y