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Meta-analysis of transcripts associated with race-specific resistance to stripe rust in wheat demonstrates common induction of blue copper-binding protein, heat-stress transcription factor, pathogen-induced WIR1A protein, and ent-kaurene synthase transcripts

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Abstract

Resistance to stripe rust in wheat is a preferred method of disease prevention. Race-specific all-stage resistance usually provides complete protection; thus an understanding of the molecular control of race-specific resistance is important. To build on previous studies of race-specific resistance controlled by the Yr5 gene, this study reports the construction and use of a custom oligonucleotide microarray to perform a meta-analysis of the transcriptional response involved in race-specific resistance conferred by Yr1, Yr5, Yr7, Yr8, Yr9, Yr10, Yr15, and Yr17. By profiling the response of eight resistance genes in a common background, we identified 28 transcripts significantly involved in the resistance phenotype across all genotypes. The most significant of these were annotated as blue copper-binding protein, heat-stress transcription factor, pathogen-induced WIR1A protein, and ent-kaurene synthase transcripts. Unique transcripts significant in each genotype were also identified, which highlighted some transcriptional events specific to certain genotypes. The approach was effective in narrowing down the list of candidate genes in comparison to studying individual genotypes. Annotation revealed key gene expression events involved in race-specific resistance. The results confirm the activity of known R-gene-mediated pathway race-specific resistance, including an oxidative burst that likely contributes to a hypersensitive response, as well as pathogenesis-related protein expression and activity of the phenylpropanoid pathway. However, several identified transcripts remained unknown and may prove interesting candidates for further characterization.

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Acknowledgments

This research was supported in part by the US Department of Agriculture, Agricultural Research Service (project no. 5348-22000-014-00D), USDA-ARS Postdoctoral Program, and Washington Wheat Commission (project no. 13C-3061-3923). PPNS no. XXXX, Department of Plant Pathology, College of Agricultural, Human, and Natural Resources Research Center, project numbers WNP00823. The authors acknowledge Lisa Orfe and Dr. Douglas Call for printing the microarrays.

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Authors and Affiliations

  1. USDA-ARS, Plant Science Research Unit, Raleigh, NC, 27695-7620, USA

    Tristan E. Coram

  2. Department of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA

    Xueling Huang, Gangming Zhan & Xianming Chen

  3. Department of Molecular Biosciences, Washington State University, Pullman, WA, 99164-4234, USA

    Matthew L. Settles

  4. USDA-ARS, Wheat Genetics, Quality, Physiology, and Disease Research Unit, Pullman, WA, 99164-6430, USA

    Xianming Chen

  5. College of Plant Protection, Northwest A&F University, Yangling, Xianyang, Shaanxi, China

    Xueling Huang & Gangming Zhan

Authors
  1. Tristan E. Coram
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  2. Xueling Huang
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  3. Gangming Zhan
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  4. Matthew L. Settles
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Corresponding author

Correspondence to Tristan E. Coram.

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Supplementary Table

The transcripts identified as uniquely significant (p < 0.10, fold change>2.0) for the incompatible interaction of each genotype in reference to mock-inoculated controls. Note that no unique transcripts were identified for genotypes Yr5 and Yr9. Functional categories were based on the Munich Information Center for Protein Sequence classifications and putative function shows the best significant BLASTX database hit from HarvEST. (DOC 125 kb)

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Coram, T.E., Huang, X., Zhan, G. et al. Meta-analysis of transcripts associated with race-specific resistance to stripe rust in wheat demonstrates common induction of blue copper-binding protein, heat-stress transcription factor, pathogen-induced WIR1A protein, and ent-kaurene synthase transcripts. Funct Integr Genomics 10, 383–392 (2010). https://doi.org/10.1007/s10142-009-0148-5

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  • DOI: https://doi.org/10.1007/s10142-009-0148-5

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