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Porcine muscle sensory attributes associate with major changes in gene networks involving CAPZB, ANKRD1, and CTBP2

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Abstract

Principal component analysis of traits related to carcass and meat properties were combined with microarray expression data for the identification of functional networks of genes and biological processes taking place during the conversion of muscle to meat. Principal components (PCs) with high loadings of meat quality traits were derived from phenotypic data of 572 animals of a porcine crossbreed population. Microarray data of 74 M. longissimus dorsi samples were correlated with PC datasets. Lists of significantly correlated genes were analyzed for enrichment of functional annotation groups as defined in the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases as well as the Ingenuity Pathways Analysis library. Ubiquitination, phosphorylation, mitochondrion dysfunction, actin, integrin, platelet-derived growth factor, epidermal growth factor, vascular endothelial growth factor, and Ca signaling pathways are correlated with meat quality. Among the significantly trait-associated genes, CAPZB, ANKRD1, and CTBP2 are promoted as candidate genes for meat quality that provide a link between the highlighted pathways. Knowledge of the relevant biological processes and the differential expression of members of the pathway will provide tools that are predictive for traits related to meat quality and that may also be diagnostic for many muscle defects or damages including muscle atrophy, dystrophy, and hypoxia.

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Acknowledgements

The authors thank Annette Jugert and Joana Bittner for the excellent technical help. This research was supported by the German Research Foundation (Deutsche Forschungsgemeinschaft, DFG; FOR 753).

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Authors and Affiliations

  1. Research Group “Functional Genome Analysis”, Research Institute for the Biology of Farm Animals (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany

    S. Ponsuksili & M. Schwerin

  2. Research Unit “Molecular Biology”, Research Institute for the Biology of Farm Animals (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany

    E. Murani & K. Wimmers

  3. Animal Breeding and Husbandry Group, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115, Bonn, Germany

    C. Phatsara & K. Schellander

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  1. S. Ponsuksili
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  2. E. Murani
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Ponsuksili, S., Murani, E., Phatsara, C. et al. Porcine muscle sensory attributes associate with major changes in gene networks involving CAPZB, ANKRD1, and CTBP2. Funct Integr Genomics 9, 455–471 (2009). https://doi.org/10.1007/s10142-009-0131-1

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