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Molecular characterization of the genomic region linked with apomixis in Pennisetum/Cenchrus

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Abstract

Apomixis is defined as asexual reproduction through seeds, although this outcome can be achieved by multiple pathways. Since little is known about the molecular control of these pathways, how they might intersect is also a mystery. Two of these pathways in the grass family, diplospory and apospory, are receiving attention from molecular biologists. Apospory in Pennisetum/Cenchrus, two genera of panicoid grasses, results in the formation of four-nucleate embryo sacs that lack antipodals. Sexual reproduction frequently aborts so that the resulting seed is composed of (1) a parthenogenetically derived embryo that is genetically identical to the mother and (2) endosperm formed through pseudogamy. The transmission of apomixis is associated with the transfer of a linkage block on a single chromosome. This linkage block contains repetitive sequences as well as hemizygous, low-copy DNA sequences. Fluorescence in situ hybridization has demonstrated that these DNA regions occur on only a single chromosome, but not its homologs, in the polyploid apomicts studied. Features of the apomixis-associated region resemble those of other chromosomal segments isolated from recombination and replete with "selfish" DNAs.

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Acknowlegements

This work has been funded by the Rockefeller Foundation, Pioneer Hi-Bred International, Limagrain SA, USDANRI (award nos. 93-37304-9363 and 99-35300-7691), and NSF (award no. 0115911). We thank Linsey Forlow for developing the illustrations in Figs. 1 and 2, and Joann Conner, Geraldine Fleming, Shailendra Goel, John McDonald, and Dominique Roche for their comments on the manuscript.

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  1. Department of Horticulture, University of Georgia Tifton Campus, Tifton, GA, 31793-0748, USA

    Peggy Ozias-Akins & Yukio Akiyama

  2. Coastal Plain Experiment Station, USDA-ARS, Tifton, GA, 31793-0748, USA

    Wayne W. Hanna

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  1. Peggy Ozias-Akins
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Correspondence to Peggy Ozias-Akins.

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Ozias-Akins, P., Akiyama, Y. & Hanna, W.W. Molecular characterization of the genomic region linked with apomixis in Pennisetum/Cenchrus . Funct Integr Genomics 3, 94–104 (2003). https://doi.org/10.1007/s10142-003-0084-8

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