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Identification of Quantitative Trait Loci for Resistance to RSIVD in Red Sea Bream (Pagrus major)

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Abstract

Red sea bream iridoviral disease (RSIVD) is a major viral disease in red sea bream farming in Japan. Previously, we identified one candidate male individual of red sea bream that was significantly associated with convalescent individuals after RSIVD. The purpose of this study is to identify the quantitative trait loci (QTL) linked to the RSIVD-resistant trait for future marker-assisted selection (MAS). Two test families were developed using the candidate male in 2014 (Fam-2014) and 2015 (Fam-2015). These test families were challenged with RSIV, and phenotypes were evaluated. Then, de novo genome sequences of red sea bream were obtained through next-generation sequencing, and microsatellite markers were searched and selected for linkage map construction. One immune-related gene, MHC class IIβ, was also used for linkage map construction. Of the microsatellite markers searched, 148 and 197 were mapped on 23 and 27 linkage groups in the female and male linkage maps, respectively, covering approximately 65% of genomes in both sexes. One QTL linked to an RSIVD-resistant trait was found in linkage group 2 of the candidate male in Fam-2014, and the phenotypic variance of the QTL was 31.1%. The QTL was closely linked to MHC class IIβ. Moreover, the QTL observed in Fam-2014 was also significantly linked to an RSIVD-resistant trait in the candidate male of Fam-2015. Our results suggest that the RSIVD-resistant trait in the candidate male was controlled by one major QTL closely linked to the MHC class IIβ gene and could be useful for MAS of red sea bream.

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Acknowledgements

We thank all the staff of Marua Suisan Co., Ltd. for collecting fish samples. We also thank Dr. Sonoko Shimizu for her kind help during the challenge tests, Mr. Shintaro Urasaki for collecting the spleens of RSIVD-infected fish, and Dr. Satoshi Kubota for useful advice on estimating genome coverage of the linkage maps. We also thank Dennis Murphy for editing this manuscript. This work was partly supported by grants from the Ehime Industrial Promotion Foundation and Ehime University.

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Authors and Affiliations

  1. R&D Division, Marua Suisan Co., Ltd., 4472 Iwagi, Kamijima-cho, Ochi-gun, Ehime, 794-2410, Japan

    Eitaro Sawayama

  2. Center for Marine Environmental Studies, Ehime University, 2-5 Bunkyo-cho, Matsuyama, Ehime, 790-8577, Japan

    Shiho Tanizawa, Shin-Ichi Kitamura & Kei Nakayama

  3. Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Kohei Ohta

  4. National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency, 422-1 Nakatsuhamaura, Minamiise-cho, Watarai-gun, Mie, 516-0193, Japan

    Akiyuki Ozaki

  5. South Ehime Fisheries Research Center, Ehime University, 1289-1 Funakoshi, Ainan-cho, Ehime, 790-8566, Japan

    Motohiro Takagi

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  1. Eitaro Sawayama
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Correspondence to Eitaro Sawayama.

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Sawayama, E., Tanizawa, S., Kitamura, SI. et al. Identification of Quantitative Trait Loci for Resistance to RSIVD in Red Sea Bream (Pagrus major). Mar Biotechnol 19, 601–613 (2017). https://doi.org/10.1007/s10126-017-9779-z

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