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Production of Tiger Puffer Takifugu rubripes Offspring from Triploid Grass Puffer Takifugu niphobles Parents

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Abstract

The tiger puffer Takifugu rubripes is one of the most popular aquacultural fish; however, there are two major obstacles to selective breeding. First, they have a long generation time of 2 or 3 years until maturation. Second, the parental tiger puffer has a body size (2–5 kg) much larger than average market size (0.6–1.0 kg). The grass puffer Takifugu niphobles is closely related to the tiger puffer and matures in half the time. Furthermore, grass puffer can be reared in small areas since their maturation weight is about 1/150 that of mature tiger puffer. Therefore, to overcome the obstacles of maturation size and generation time of tiger puffer, we generated surrogate grass puffer that can produce tiger puffer gametes through germ cell transplantation. Approximately 5000 tiger puffer testicular cells were transplanted into the peritoneal cavity of triploid grass puffer larvae at 1 day post hatching. When the recipient fish matured, both males and females produced donor-derived gametes. Through their insemination, we successfully produced donor-derived tiger puffer offspring presenting the same body surface dot pattern, number of dorsal fin rays, and DNA fingerprint as those of the donor tiger puffer, suggesting that the recipient grass puffer produced functional eggs and sperm derived from the donor tiger puffer. Although fine tunings are still needed to improve efficiencies, surrogate grass puffer are expected to accelerate the breeding process of tiger puffer because of their short generation time and small body size.

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Funding Information

This study was partly supported by a Grant-in-Aid for Scientific Research (KAKENHI) (25114005) on Innovative Areas, “Mechanisms regulating gamete formation in animals,” and the Ocean Resource Use Promotion Technology Development Program conducted by MEXT.

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Authors and Affiliations

  1. Nagasaki Prefectural Institute of Fisheries, 1551-4 Taira, Nagasaki-shi, Nagasaki, 851-2213, Japan

    Masaomi Hamasaki, Souta Yoshikawa, Kazushi Kadomura, Toshiyuki Yamada & Kadoo Miyaki

  2. Division of Fisheries Resource and Sciences, Faculty of Fisheries, Kagoshima University, 4-50-20 Shimoarata, Kagoshima-shi, Kagoshima, 890-0056, Japan

    Yutaka Takeuchi

  3. Department Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo, 108-8477, Japan

    Ryosuke Yazawa & Goro Yoshizaki

  4. Fisheries Laboratory, Graduate School of Agricultural and Life Sciences, University of Tokyo, 2971-4 Bentenjima, Maisaka, Hamamatsu-shi, Shizuoka, 431-0214, Japan

    Kiyoshi Kikuchi

Authors
  1. Masaomi Hamasaki
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  2. Yutaka Takeuchi
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  3. Ryosuke Yazawa
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  4. Souta Yoshikawa
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  5. Kazushi Kadomura
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  6. Toshiyuki Yamada
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  7. Kadoo Miyaki
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  8. Kiyoshi Kikuchi
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  9. Goro Yoshizaki
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Corresponding author

Correspondence to Masaomi Hamasaki.

Additional information

Triploid grass puffer that received tiger puffer germ cells intraperitoneally successfully produced donor-derived tiger puffer eggs and sperm.

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Hamasaki, M., Takeuchi, Y., Yazawa, R. et al. Production of Tiger Puffer Takifugu rubripes Offspring from Triploid Grass Puffer Takifugu niphobles Parents. Mar Biotechnol 19, 579–591 (2017). https://doi.org/10.1007/s10126-017-9777-1

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  • DOI: https://doi.org/10.1007/s10126-017-9777-1

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