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Immunostimulatory Effect of Enzyme-Modified Hizikia fusiformein a Mouse Model In Vitro and Ex Vivo

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Abstract

Hizikia fusiforme, a brown seaweed, has been utilized as a health food and in traditional medicine. In this study, we investigated whether enzyme-modified H. fusiforme extracts (EH) have immunological effects compared with normal H. fusiforme extracts (NH). The effects of NH and EH on immune responses were investigated by assessing nitric oxide (NO) production, phagocytosis, and cytokine secretion in RAW 264.7 murine macrophages and mice. Also, fucosterol was evaluated to find the active component of NH and EH by addressing cytotoxicity test and NO production. Both of NH and EH significantly increased cell viability and NO synthesis. Tumor necrosis factor-α (TNF-α) expression was more induced by EH with LPS treatment. Phagocytic activity, as the primary function of macrophages, was markedly induced by EH treatment. Additionally, EH encouraged splenocyte proliferation and recovered the levels of cytokines IL-1β, IL-6, and TNF-α in mice. Finally, fucosterol increased NO production with no cytotoxicity, which means that fucosterol is an active component of EH. In conclusion, EH has the potential to modulate immune function and could offer positive therapeutic effect for immune system diseases.

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Acknowledgements

The authors would like to thank YTH LifeScience LTD.

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Authors and Affiliations

  1. Department of Oriental Medicinal Materials & Processing, College of Life Science, Kyung Hee University, Yongin-si, South Korea

    Sang-Yong Park, Eunson Hwang, Yu-Kyong Shin & Tae-Hoo Yi

  2. Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-Si, South Korea

    Don-Gil Lee, Jung-Eun Yang, Jae-Hee Park & Tae-Hoo Yi

Authors
  1. Sang-Yong Park
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  2. Eunson Hwang
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  3. Yu-Kyong Shin
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  4. Don-Gil Lee
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  5. Jung-Eun Yang
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  6. Jae-Hee Park
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  7. Tae-Hoo Yi
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Correspondence to Tae-Hoo Yi.

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The experimental protocol [KHUASP(SE)-15-027] was approved by the Institutional Animal Care and Use Committee of Kyung Hee University.

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The authors declare that they have no conflict of interest.

Additional information

Sang-Yong Park and Eunson Hwang contributed equally to this work.

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Park, SY., Hwang, E., Shin, YK. et al. Immunostimulatory Effect of Enzyme-Modified Hizikia fusiformein a Mouse Model In Vitro and Ex Vivo. Mar Biotechnol 19, 65–75 (2017). https://doi.org/10.1007/s10126-017-9727-y

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  • DOI: https://doi.org/10.1007/s10126-017-9727-y

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