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Characterization of Carrageenan Extracted from Hypnea bryoides in Oman

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Abstract

Carrageenophyte red seaweed from Oman, Hypnea bryoides, extracted using three different processes: an aqueous, a mild alkaline, and a more vigorous alkaline extraction was investigated. The resulting extract precipitated by alcohol was subject to chemical and rheological measurements. The total carbohydrate [ranged from 36.78 to 41.65 g/100 g], and ash [39.04 to 43.11 g/100 g] were the most abundant components in H. bryoides and contrary to the two, lipid content was found at a minimum [ranging from 2.95 to 3.38 g/100 g]. Alkali treatment with NaOH allowed complete conversion of kappa (κ) carrageenan form as detected by FTIR analysis. Total yield by alkali treatments gave higher yields (33%) compared with aqueous treatments (12%). However, subsequent aqueous treatment produced mixed carrageenan (μ and κ) with higher molecular weight compared with the alkali treatments which produced single carrageenan form (κ) with molecular weight of 4.1 × 105 Da. The effects of thermal history on gel–sol and sol–gel transition were investigated by differential scanning calorimeter (DSC) and rheology on a pure sample and 1.5% κ-carrageenan mixture added with 30 mM KCl. Transition temperatures from DSC and rheology showed comparable results and were in good agreement with those previously reported.

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Acknowledgment

This work was supported by HM Sultan Qaboos Trust Fund (SR/AGR/FOOD/05/01, PI: B Soussi), University of Gothenburg and the Swedish Research Council (K2005-73-00536, PI: B. Soussi). The authors thank Barry P. Jupp for the valuable discussions; Rabea Al Maqbaly, and Suleiman Al Sheili for their technical assistance.

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Authors and Affiliations

  1. College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat, Oman

    Ahmed Ali Al-Alawi, Insaaf Mohammed Al-Marhubi, Mohammed Said Moosa Al-Belushi & Bassam Soussi

  2. UNESCO Chair, Sultan Qaboos University, Muscat, Oman

    Bassam Soussi

  3. Institute for Clinical Science, Sahlgrens Academy, University of Gothenburg, Gothenburg, Sweden

    Bassam Soussi

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  1. Ahmed Ali Al-Alawi
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  2. Insaaf Mohammed Al-Marhubi
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  3. Mohammed Said Moosa Al-Belushi
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  4. Bassam Soussi
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Correspondence to Bassam Soussi.

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Al-Alawi, A.A., Al-Marhubi, I.M., Al-Belushi, M.S.M. et al. Characterization of Carrageenan Extracted from Hypnea bryoides in Oman. Mar Biotechnol 13, 893–899 (2011). https://doi.org/10.1007/s10126-010-9350-7

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  • DOI: https://doi.org/10.1007/s10126-010-9350-7

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