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Construction and Characterization of the BAC Library for Common Carp Cyprinus Carpio L. And Establishment of Microsynteny with Zebrafish Danio Rerio

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An Erratum to this article was published on 16 August 2011

Abstract

A bacterial artificial chromosome (BAC) library of common carp Cyprinus carpio L. was constructed as a part of ongoing common carp genome project, which is aiming assembly of common carp genome. The library, containing a total of 92,160 BAC clones with an average insert size of 141 kb, was constructed into the restriction site of Hind III on BAC vector CopyControl pCC1BAC, covering 7.7 X haploid genome equivalents. Three dimension pools and superpools of the BAC library were established and 23 positive clones of 14 targets were identified from one-fifth of the BAC library. Pilot project of BAC end sequencing was conducted on 2,688 BAC ends from 1,344 clones and harvested 2,522 high-quality Q20 sequences with average length of 677 bp. The sequencing success rate was 93.8% and pair-end success rate was 92.3%. A total of 212 microsyntenies had been established between common carp and zebrafish genomes as a trial for genome-wide comparative genomics in these two closely related species.

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Acknowledgments

This study was supported by the grants from National Department Public Benefit Research Foundation (award no. 200903045), “948” program of Minister of Agriculture of China (award no. 2010-Z11), and Research Foundation of Chinese Academy of Fishery Sciences (award no. 2009B002). We thank Mr. Dingchen Cao and Mr. Youyi Kuang in Heilongjiang Fishery Research Institute, Chinese Academy of Fishery Sciences, for their assistant on fish sampling.

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Authors and Affiliations

  1. The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing, 100141, China

    Yan Li, Peng Xu, Zixia Zhao, Jian Wang, Yan Zhang & Xiao-Wen Sun

  2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China

    Yan Li

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  1. Yan Li
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  2. Peng Xu
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  3. Zixia Zhao
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  4. Jian Wang
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  5. Yan Zhang
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Correspondence to Peng Xu.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s10126-011-9404-5

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Li, Y., Xu, P., Zhao, Z. et al. Construction and Characterization of the BAC Library for Common Carp Cyprinus Carpio L. And Establishment of Microsynteny with Zebrafish Danio Rerio . Mar Biotechnol 13, 706–712 (2011). https://doi.org/10.1007/s10126-010-9332-9

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