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Purification of Sinus Gland Peptides Having Vitellogenesis-Inhibiting Activity from the Whiteleg Shrimp Litopenaeus Vannamei

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Abstract

Vitellogenesis-inhibiting hormone (VIH) in Crustacea belongs to the crustacean hyperglycemic hormone (CHH)-family. To characterize multiple VIH molecules in the whiteleg shrimp Litopenaeus vannamei, seven CHH-family peptides designated as Liv-SGP-A, -B, -C, -D, -E, -F, and -G were purified by reversed-phase HPLC and identified by N-terminal amino acid sequencing. The dose-response effects of these peptides on vitellogenin mRNA levels were examined using in vitro incubation of ovarian fragments of the kuruma prawn Marsupenaeus japonicus. Liv-SGP-D showed no significant inhibitory activities, while the other six peptides significantly reduced vitellogenin mRNA levels, however, with differing efficacies, in the order of Liv-SGP-C, -F, -G > -A, -B > -E. Liv-SGP-G was the most abundant CHH-family peptide in the sinus gland and showed strong vitellogenesis-inhibiting activity. As a result of detailed structural analysis, its complete primary structure was determined; it consisted of 72 amino acid residues and possesses an amidated C-terminus.

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Acknowledgments

We thank Dr. V. Jayasankar of the Japan International Research Center for Agricultural Sciences for helpful suggestions. T.O. was supported by the Cooperative System for Supporting Priority Research of the Japan Science and Technology Corporation. This work was supported by the Research and Development Program for New Bio-industry Initiatives of the Bio-oriented Technology Research Advancement Institution (BRAIN) of Japan.

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Authors and Affiliations

  1. Japan International Research Center for Agricultural Sciences, 1-1 Ohwashi, Tsukuba, Ibaraki, 305-8686, Japan

    Naoaki Tsutsui, Tsuyoshi Ohira, Ichiro Kawazoe & Marcy N. Wilder

  2. School of Fisheries Sciences, Kitasato University, Sanriku, Ofunato, Iwate, 022-0101, Japan

    Akiyoshi Takahashi

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  1. Naoaki Tsutsui
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  2. Tsuyoshi Ohira
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  3. Ichiro Kawazoe
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  4. Akiyoshi Takahashi
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  5. Marcy N. Wilder
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Correspondence to Marcy N. Wilder.

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Tsutsui and Ohira contributed equally to this work.

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Tsutsui, N., Ohira, T., Kawazoe, I. et al. Purification of Sinus Gland Peptides Having Vitellogenesis-Inhibiting Activity from the Whiteleg Shrimp Litopenaeus Vannamei . Mar Biotechnol 9, 360–369 (2007). https://doi.org/10.1007/s10126-006-6151-0

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