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Mitochondrial DNA Sequence and Gene Organization in the Australian Blacklip Abalone Haliotis rubra (Leach)

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An Erratum to this article was published on 05 October 2006

Abstract

The complete mitochondrial DNA of the blacklip abalone Haliotis rubra (Gastropoda: Mollusca) was cloned and 16,907 base pairs were sequenced. The sequence represents an estimated 99.85% of the mitochondrial genome, and contains 2 ribosomal RNA, 22 transfer RNA, and 13 protein-coding genes found in other metazoan mtDNA. An AT tandem repeat and a possible C-rich domain within the putative control region could not be fully sequenced. The H. rubra mtDNA gene order is novel for mollusks, separated from the black chiton Katharina tunicata by the individual translocations of 3 tRNAs. Compared with other mtDNA regions, sequences from the ATP8, NAD2, NAD4L, NAD6, and 12S rRNA genes, as well as the control region, are the most variable among representatives from Mollusca, Arthropoda, and Rhynchonelliformea, with similar mtDNA arrangements to H. rubra. These sequences are being evaluated as genetic markers within commercially important Haliotis species, and some applications and considerations for their use are discussed.

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Acknowledgments

We thank Amber Stephens for providing pBeloBAC II prepared for cloning HindIII-cut mtDNA. We thank Adam Miller Dr. Chris Burridge, and others from the School of Ecology and the Environment and the School of Biological and Chemical Sciences, Deakin University, for assistance with sequence analysis and critical review of this manuscript. Ben Maynard is supported by a Deakin University Postgraduate Scholarship.

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  1. School of Biological & Chemical Sciences, Deakin University, Geelong, VIC 3217, Australia

    Ben T. Maynard, Lyndal J. Kerr, Joanne M. McKiernan, Eliza S. Jansen & Peter J. Hanna

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  1. Ben T. Maynard
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  2. Lyndal J. Kerr
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  3. Joanne M. McKiernan
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  4. Eliza S. Jansen
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Correspondence to Peter J. Hanna.

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An erratum to this article is available athttp://dx.doi.org/10.1007/s10126-005-5704-y.

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Maynard, B.T., Kerr, L.J., McKiernan, J.M. et al. Mitochondrial DNA Sequence and Gene Organization in the Australian Blacklip Abalone Haliotis rubra (Leach). Mar Biotechnol 7, 645–658 (2005). https://doi.org/10.1007/s10126-005-0013-z

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