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Polymorphism in Nucleotide Sequence of Mitochondrial Intergenic Region in Scleractinian Coral (Galaxea fascicularis)

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Abstract

A region of 826 bp that is unlikely to code for a protein, ribosomal RNA, or transfer RNA was identified between the cytochrome b and NADH–ubiquinone oxidoreductase chain 2 loci in the mitochondrial DNA of the scleractinian reef coral Galaxea fascicularis. Nucleotide sequences were determined in a part (625 bp) of this intergenic region in 95 individuals collected at 9 sites in the Ryukyu Archipelago in southwestern Japan. A total of 8 haplotypes were found, and a deletion of 290 bp was found in 3 of them. Significant differences were found in frequencies of the haplotypes at 3 sampling sites. The presence or absence of the deletion was highly correlated with the hard or soft morphotype. The deletion was found in the majority of hard-type colonies, but in a small fraction of soft-type individuals.

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References

  • C.T. Beagley R. Okimoto D.R. Wolstenholme (1998) ArticleTitleThe mitochondrial genome of the sea anemone Metridium senile (Cnidaria): introns, a paucity of tRNA genes, and a near-standard genetic code Genetics 148 1091–1108 Occurrence Handle1:CAS:528:DyaK1cXks1eit7o%3D Occurrence Handle9539427

    CAS  PubMed  Google Scholar 

  • K.A. Beauchamp D.A. Powers (1996) ArticleTitleSequence variation of the first internal spacer (ITS-1) of ribosomal DNA in ahermatypic corals from California Mol Mar Biol Biotechnol 5 357–362 Occurrence Handle1:CAS:528:DyaK2sXivVSnuw%3D%3D Occurrence Handle8983201

    CAS  PubMed  Google Scholar 

  • C.A. Chen J.K. Yu (2000) ArticleTitleUniversal primers for amplification of mitochondrial small subunit ribosomal RNA–encoding gene in Scleractinian corals Mar Biotechnol 2 146–153 Occurrence Handle10.1007/s101260000040 Occurrence Handle1:CAS:528:DC%2BD3cXjtF2jtro%3D Occurrence Handle10811953

    Article  CAS  PubMed  Google Scholar 

  • H. Fukami M. Omori M. Hatta (2000) ArticleTitlePhylogenetic relationships in the coral family Acroporidae, reassessed by inference from mitochondrial genes Zool Sci 17 689–696 Occurrence Handle10.2108/zsj.17.689 Occurrence Handle1:CAS:528:DC%2BD3cXmslWktb4%3D

    Article  CAS  Google Scholar 

  • M. Hidaka (1992) ArticleTitleUse of nematocyst morphology for taxonomy of some related species of scleractinian corals Galaxea 11 21–28

    Google Scholar 

  • Kusen, J.D. (1987). Intraspecific variation of a scleractinian coral, Galaxea fascicualris (Linnaeus). Master’s thesis, University of the Ryukyus

  • E. Maier R. Tollrian B. Nurnberger (2001) ArticleTitleDevelopment of species-specific markers in an organism with endosymbionts: microsatellites in the scleractinian coral Seriatopora hystrix Mol Ecol Notes 1 157–159 Occurrence Handle10.1046/j.1471-8278.2001.00058.x Occurrence Handle1:CAS:528:DC%2BD3MXnslKku7o%3D

    Article  CAS  Google Scholar 

  • D.M. Odorico D.J. Miller (1997) ArticleTitleVariation in the ribosomal internal transcribed spacers and 5.8S rDNA among five species of Acropora (Cnidaria, Scleractinia): patterns of variation consistent with reticulate evolution Mol Biol Evol 14 465–473 Occurrence Handle1:CAS:528:DyaK2sXjtVGnsLg%3D Occurrence Handle9159924

    CAS  PubMed  Google Scholar 

  • W.R. Pearson D.J. Lipman (1988) ArticleTitleImproved tools for biological sequence comparison Proc Natl Acad Sci U S A 85 2444–2448 Occurrence Handle1:CAS:528:DyaL1cXktFyit78%3D Occurrence Handle3162770

    CAS  PubMed  Google Scholar 

  • M. Raymond F. Rousset (1995) ArticleTitleGENEPOP (Version 1.2): population-genetics software for exact tests and ecumenicism J Hered 86 248–249

    Google Scholar 

  • M. Rodriguez-Lanetty O. Hoegh-Guldberg (2002) ArticleTitleThe phylogeography and connectivity of the latitudinally widespread scleractinian coral Plesiastrea versipora in the Western Pacific Mol Ecol 11 1177–1189 Occurrence Handle10.1046/j.1365-294X.2002.01511.x Occurrence Handle1:CAS:528:DC%2BD38Xmt1ehu70%3D Occurrence Handle12074725

    Article  CAS  PubMed  Google Scholar 

  • S.L. Romano S.R. Palumbi (1996) ArticleTitleEvolution of scleractinian corals inferred from molecular systematics Science 271 640–642 Occurrence Handle1:CAS:528:DyaK28XptVyiug%3D%3D

    CAS  Google Scholar 

  • S.L. Romano S.R. Palumbi (1997) ArticleTitleMolecular evolution of a portion of the mitochondrial 16S ribosomal gene region in scleractinian corals J Mol Evol 45 397–411 Occurrence Handle1:CAS:528:DyaK2sXms1Wmu7g%3D Occurrence Handle9321419

    CAS  PubMed  Google Scholar 

  • Schneider, S., Roessli, D, and Excoffier, L. (2000). Arlequin Version 2000: A Software for Population Genetics Data Analysis. Geneva, Switzerland: Genetics and Biometry Laboratory, University of Geneva (available at http://anthro.unige.ch/arlequin )

  • T.L. Shearer M.J.H. Oppen Particlevan S.L. Romano G. Worheide (2002) ArticleTitleSlow mitochondrial DNA sequence evolution in the Anthozoa (Cnidaria) Mol Ecol 11 2475–2487 Occurrence Handle10.1046/j.1365-294X.2002.01652.x Occurrence Handle1:CAS:528:DC%2BD38Xpsl2ms7g%3D Occurrence Handle12453233

    Article  CAS  PubMed  Google Scholar 

  • M.J.H. Oppen Particlevan B.L. Willis H.W.J.A. Vugt Particlevan D.J. Miller (2000a) ArticleTitleExamination of species boundaries in the Acropora cervicornis group (Scleractinia, Cnidaria) using nuclear DNA sequence analyses Mol Evol 9 1363–1373 Occurrence Handle10.1046/j.1365-294x.2000.01010.x Occurrence Handle1:STN:280:DC%2BD3M%2FgvF2msg%3D%3D

    Article  CAS  Google Scholar 

  • M.J.H. Oppen Particlevan J. Catmull B.J. McDonald N.R. Hislop P.J. Hagerman D.J. Miller (2000b) ArticleTitleThe mitochondrial genome of Acropora tenuis (Cnidaria; Scleractinia) contains a large group I intron and a candidate control region J Mol Evol 55 1–13 Occurrence Handle10.1007/s00239-001-0075-0

    Article  Google Scholar 

  • M.J.H. Oppen Particlevan B.L. Willis T. Rheede Particlevan D.J. Miller (2002) ArticleTitleSpawning times, reproductive compatibilities and genetic structuring in the Acropora aspera group: evidence for natural hybridization and semi-permeable species boundaries in corals Mol Ecol 11 1363–1376 Occurrence Handle10.1046/j.1365-294X.2002.01527.x Occurrence Handle12144658

    Article  PubMed  Google Scholar 

  • J.E.N. Veron (2000) Corals of the World Australian Institute of Marine Sciences Townsville, Australia

    Google Scholar 

  • Yamazato, K. (1988). Reproductive mechanism of Galaxea fascicularis. In: Report for Grant-in-Aid on “Early phase of reproduction of marine resource organisms.” Ministry of Education, Science and Culture, Japan, 148–155 (in Japanese)

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Acknowledgments

The authors are grateful to Drs. Mineo Okamoto (Tokyo University of Marine Science and Technology) and Satoshi Nojima (Kyushu University), and Messrs. Yoshikatsu Nakano (University of the Ryukyus) and Kenji Iwao (Akajima Marine Science Laboratory), for their help in collecting coral samples, and to Mr. Hirohiko Takeshima for help in the statistical analysis. This work was supported in part by a scholarship from Nippon Life Insurance Foundation, a Grant-in-aid (12NP0201) from the Ministry of Education, Science, Sports and Culture, Japan, and funding on the program “Construct the genetic resource library of unidentified microbes based on genome information” from New Energy and Industrial Technology Development Organization. Coral samples were collected with the permission of the Okinawa prefectural government.

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Authors and Affiliations

  1. Division of Marine Biosciences, Ocean Research Institute, University of Tokyo, 1-15-1 Minamidai, Nakano, Tokyo, 164-8639, Japan

    Toshiki Watanabe & Mutsumi Nishida

  2. Department of Zoology, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan

    Katsutoshi Watanabe

  3. Department of Chemistry, Biology and Marine Sciences, Faculty of Science, University of the Ryukyus, 1-Senbaru, Nishihara-cho, Okinawa, 903-0213, Japan

    Defny S. Wewengkang & Michio Hidaka

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  1. Toshiki Watanabe
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  2. Mutsumi Nishida
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  3. Katsutoshi Watanabe
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  5. Michio Hidaka
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Correspondence to Toshiki Watanabe.

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Watanabe, T., Nishida, M., Watanabe, K. et al. Polymorphism in Nucleotide Sequence of Mitochondrial Intergenic Region in Scleractinian Coral (Galaxea fascicularis). Mar Biotechnol 7, 33–39 (2005). https://doi.org/10.1007/s10126-004-3200-4

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  • DOI: https://doi.org/10.1007/s10126-004-3200-4

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