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Fish as Bioreactors: Transgene Expression of Human Coagulation Factor VII in Fish Embryos

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Abstract

A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.

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Author notes

  1. Ferenc Müller

    Present address: Institute of Toxicology and Genetics, Forschungszentrum Karlsruhe, D-76021, Karlsruhe, Germany

  2. M. Aziz. Rahman

    Present address: School of Chemical and Life Sciences, University of Greenwich at Medway, Central Avenue, Chatham, Kent, ME4 4TB, U.K.

  3. Darren W. Williams

    Present address: Department of Biology, University of Washington, Seattle, WA 99185-1800, U.S.A.

Authors and Affiliations

  1. Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, SO16 7PX, U.K.

    Gyulin Hwang, Ferenc Müller, M. Aziz. Rahman, Darren W. Williams, Hamid Farahmand & Norman Maclean

  2. Haemophilia and Haemostasis Centre, Royal Free and UCL Medical School, Rowland Hill St., London, NW3 2PF, U.K.

    Paul J. Murdock & K. John Pasi

  3. Department of Surgery, Royal Free and UCL Medical School, Rowland Hill St., London, NW3 2PF, U.K.

    Geoffrey Goldspink

Authors
  1. Gyulin Hwang
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  2. Ferenc Müller
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  7. Geoffrey Goldspink
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  8. Hamid Farahmand
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  9. Norman Maclean
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Correspondence to Norman Maclean.

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Hwang, G., Müller, F., Rahman, M.A. et al. Fish as Bioreactors: Transgene Expression of Human Coagulation Factor VII in Fish Embryos. Mar Biotechnol 6, 485–492 (2004). https://doi.org/10.1007/s10126-004-3121-2

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  • DOI: https://doi.org/10.1007/s10126-004-3121-2

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