Abstract
In order to study the genetic structure of the Adriatic shared stock of red mullet (Mullus barbatus), we developed a set of dinucleotide microsatellite markers. A dinucleotide-enriched genomic library was obtained, and 6 polymorphic dinucleotide loci were successfully optimized. The markers showed high expected heterozygosity (from 0.68 to 0.92) and allele number (from 12 to 33); thus they appear to be suitable for detecting genetic differences in the population of red mullet. Four Adriatic samples were subsequently analyzed for microsatellite variation, and the results showed subtle but statistically significant genetic differentiation, indicating that the Adriatic red mullet may group into local, genetically isolated populations. No correlation between geographic distance and genetic differentiation was observed. In addition, the evidence of recent bottlenecks in the Adriatic samples indicates that the observed population subdivision might reflect random local allelic variations, generated by reproductive success, survival rates, or fishing pressure.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
AdriaMed (2000). Priority Topics Related to Shared Demersal Fishery Resources of the Adriatic Sea. Report of the First Meeting of the AdriaMed Working Group on Shared Demersal Resources. GCP/RER/010/ITA-02, 1-21. Rome, Italy: FAO-MiPAF Scientific Cooperation to Support Responsible Fisheries in the Adriatic Sea, 1–21 Available at http://www.faoadriamed.org/html/av-docurnents.html.
M.A. Banks W. Eichert (2000) ArticleTitleWHICHRUN (Version 3.2): a computer program for population assignment of individuals based on multilocus genotype data J Hered 91 87–89
Belkhir, K., Borsa, P., Chikhi, L., Raufaste, N., and Bonhomme, F. (1996). Genetix 4.04, logiciel sous Windows TM pour la génétique des populations. Montpellier, France: Laboratoire Génome, Populations, Interactions, CNRS UMR 5000, Université de Montpellier II.
J.M. Cornuet G. Luikart (1996) ArticleTitleDescription and power analysis of two tests for detecting recent population bottlenecks from allele frequency data Genetics 144 2001–2014 Occurrence Handle1:STN:280:ByiC3s3mtlU%3D Occurrence Handle8978083
J.A. Woody ParticleDe J.C. Avise (2000) ArticleTitleMicrosatellite variation in marine, freshwater and anadromous fishes compared with other animals J Fish Biol 56 461–473
A. Estoup F. Rousset Y. Michalakis J.M. Cornuet M. Adriamanga R. Guyomard (1998) ArticleTitleComparative analysis of microsatellite and allozyme markers: a case study investigating microgeographic differentiation in brown trout (Salmo trutta) Mol Ecol 7 339–353
F. Garoia I. Guarniero F. Tinti (2003) ArticleTitlePolymorphic dinucleotide microsatellites for the Mediterranean angler species (Lophiidae) Mol Ecol Notes 3 294–296
O.J. Hardy X. Vekemans (2002) ArticleTitleSPAGeDi: a versatile computer program to analyse spatial genetic structure at the individual or population levels Mol Ecol Notes 2 618–620
L. Hauser G.J. Adcock P.J. Smith J. Bernal Ramirez G.R. Carvalho (2002) ArticleTitleLoss of microsatellite diversity and low effective population size in an overexploited population of New Zealand snapper Proc Natl Acad Sci U S A 99 11742–11747
P.W. Hedrick (1999) ArticleTitleHighly variable loci and their interpretation in evolution and conservation [Perspective] Evolution 53 313–318
G. Hoarau AD. Rijnsdorp H.W. Veer ParticleVan der W.T. Stam J.L. Olsen (2002) ArticleTitlePopulation structure of plaice (Pleuronectes platessa L.) in northern Europe: microsatellites revealed large-scale spatial and temporal homogeneity Mol Ecol 11 1165–1176
J.A. Hutchings (2000) ArticleTitleCollapse and recovery of marine fishes Nature 406 882–885
H. Knutsen P.E. Jorde C. Andre N.C. Stenseth (2003) ArticleTitleFine-scaled geographical population structuring in a highly mobile marine species: the Atlantic cod Mol Ecol 12 385–394
G. Manfrin M. Paolini C. Piccinetti (1998) ArticleTitleLe risorse demersali dell’Alto e Medio Adriatico Biol Mar Medit 5 IssueID3 96–108
G. Marano N. Ungano C.A. Marsan R. Marano (1988) ArticleTitleLa ricerca sulle risorse demersali del bacino adriatico sud-occidentale (anni 1985–97): sintesi dei risultati Biol Mar Medit 5 IssueID3 109–119
S.A. Miller D.D. Dykes H.F. Polesky (1988) ArticleTitleA simple salting out procedure for extracting DNA from human nucleated cells Nucleic Acids Res 16 1215 Occurrence Handle1:CAS:528:DyaL1cXhsVKlsrs%3D Occurrence Handle3344216
C.L. Nesbø E.K. Rueness S.A. Iversen D.W. Skagen K.S. Jakobsen (2000) ArticleTitlePhylogeography and population history of Atlantic mackerel (Scomber scombrus L.): a genealogical approach reveals genetic structuring among the eastern Atlantic stocks Proc R Soc Lond B Biol Sci 7 281–292 Occurrence Handle10.1098/rspb.2000.0998
M. Raymond F. Rousset (1995) ArticleTitleGENEPOP (Version 1.2.): population genetics software for exact tests and ecumenicism J Hered 86 248–249
G. Relini J. Bertrand A. Zamboni (1999) ArticleTitleSynthesis of the knowledge on bottom fishery resources in central Mediterranean (Italy and Corsica) Biol Mar Medit 6 IssueIDsuppl 1 276–291
W.R. Rice (1989) ArticleTitleAnalyzing tables of statistical tests Evolution 43 223–225
Rozen, S., and Skaletsky, H.J. (1998). PRIMER3. Available at http://wwwgenome.wi.mit.edu/genome_software/other/primer3.html.
D.E. Ruzzante C.T. Taggart D. Cook (1998) ArticleTitleA nuclear DNA basis for shelf- and bank-scale population structure in northwest Atlantic cod (Gadus morhua): Labrador to Georges Bank Mol Ecol 7 1663–1680
M. Slatkin (1993) ArticleTitleIsolation by distance in equilibrium and non-equilibrium populations Evolution 47 264–279
D. Tautz (1989) ArticleTitleHypervariability of simple sequences as a general source for polymorphic DNA markers Nucleic Acids Res 17 6463–6471 Occurrence Handle1:CAS:528:DyaL1MXls1ylt7Y%3D Occurrence Handle2780284
B. Waldman J.S. McKinnon (1993) Inbreeding and outbreeding in fishes, amphibians and reptiles N.W. Thornbel (Eds) The Natural History of Inbreeding and Outbreeding University of Chicago Press Chicago, Ill 250–282
B.S. Weir C.C. Cockerham (1984) ArticleTitleEstimating F-statistics for the analysis of population structure Evolution 38 1358–1370
L. Zane L. Bargelloni T. Patarnello (2002) ArticleTitleStrategies for microsatellite isolation: a review Mol Ecol 11 1–16
Acknowledgments
We thank Nicola Ungaro (Marine Biology Lab of Bari, Italy) and the staff of the Marine Biology & Fishery Lab of Fano, Italy, for providing samples. This work is a part of the GenPopAdr project financed by the Ministero Italiano delle Politiche Agricole e Forestali within the framework of the VI Piano Triennale della Pesca e Acquacoltura-Risorse Biologiche (Project 6-A-24).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Garoia, F., Guarniero, I., Piccinetti, C. et al. First Microsatellite Loci of Red Mullet (Mullus barbatus) and Their Application to Genetic Structure Analysis of Adriatic Shared Stock. Mar Biotechnol 6, 446–452 (2004). https://doi.org/10.1007/s10126-004-3045-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10126-004-3045-x