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Isolation of the Promoters of Atlantic Salmon MHCII Genes

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Abstract

The major histocompatibility complex class II (MHCII) has a central role in the immune response of vertebrates with its function of presenting antigenic peptides to the T-cell receptors. We have isolated the promoters and intron 1 of MHCIIα and MHCIIβ genes of Atlantic salmon. To isolate these promoters, we constructed an Atlantic salmon (Salmo salar) promoter finder kit (analogous to the commercially available “human promoter finder kit”). By nucleotide sequence alignment of known MHCII promoter regions, we identified the 3 conserved regulatory X, X2, and Y boxes in the salmon promoters. The W box was not found. In contrast, a salmon-specific putative W box was identified. Both of the isolated Atlantic salmon MHCIIα and β promoters (included in patent applications by Genomar A/S, Oslo, Norway) were found to be functional since they both gave positive yellow fluorescence protein signal when inserted as promoters in the pEYFP-1 reporter plasmid and transfected into the salmon head kidney cell line (SHK-1).

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Acknowledgements

We thank Dr. Unni Grimholt for providing us with the salmon MHCIIα nucleotide sequence before publication. We also thank Dr. Birgit Dannevig for providing us with the SHK-1 cells. This project was funded by the Norwegian Research Council. Atlantic salman MHCIIα and MHCIIβ promoters are included in patent applications by Genomer A/S, Oslo, Norway.

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Author notes

  1. Birthe Mikkelsen

    Present address: Institutt for kirurgisk forskning, Songsvannsveien 20, Rikshospitalet, 0027 Oslo, Norway

  2. Maria Lundin

    Present address: Natural Södertörns University College, Box 4101, 141 04 Huddinge, Sweden

Authors and Affiliations

  1. Department of Morphology, Genetics and Aquatic Biology, Norwegian School of Veterinary Science, P.O. Box. 8146-Dep, N-0033 Oslo, Norway

    Mohasina Syed, Olav Vestrheim, Birthe Mikkelsen & Maria Lundin

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  1. Mohasina Syed
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  2. Olav Vestrheim
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  3. Birthe Mikkelsen
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  4. Maria Lundin
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Correspondence to Mohasina Syed.

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Syed, M., Vestrheim, O., Mikkelsen, B. et al. Isolation of the Promoters of Atlantic Salmon MHCII Genes . Mar. Biotechnol. 5, 253–260 (2003). https://doi.org/10.1007/s10126-002-0063-4

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  • DOI: https://doi.org/10.1007/s10126-002-0063-4

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