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Prospecting exopolysaccharides produced by selected bacteria associated with marine organisms for biotechnological applications

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Abstract

In this study, bacteria associated with marine organisms were screened for the production of exopolysaccharides (EPSs) on MY media containing sea salts (2.5%–10%). Three selected isolates were identified as Alteromonas sp. PRIM-21, Nitratireductor sp. PRIM-24 and Enterobacter sp. PRIM-26 using 16S rRNA gene sequencing. Optimization of the growth and EPS production kinetics in relation to incubation time were assessed. The purified EPS yield was 590, 650 and 540 mg·L−1 culture media respectively in Alteromonas sp. PRIM-21, Nitratireductor sp. PRIM-24 and Enterobacter sp. PRIM-26. Biochemical and FTIR analyses revealed the presence of biologically important functional groups in the EPS produced by all the three isolates. The EPS produced by Nitratireductor sp. PRIM-24 and Alteromonas sp. PRIM-21 showed 2.0% sulfate content. These bacterial EPS also showed antioxidant and emulsifying activities and the EPS produced by Enterobacter sp.PRIM-26 showed significantly higher antioxidant activities in terms of superoxide (IC50 0.33 mg·mL−1) and DPPH (IC50 0.44 mg·mL−1) radical scavenging. It also showed higher emulsifying activities against selected hydrophobic substrates with EI24 values above 60%. From the results of the study, it can be concluded that the isolated bacteria produce EPS that can be investigated in detail for biotechnological applications.

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Authors and Affiliations

  1. Yenepoya Research Centre, Yenepoya University, Deralakatte, Mangalore, Karnataka, India

    P. Priyanka, A. B. Arun & P. D. Rekha

  2. Department of Soil and Environment Sciences, National Chung-Hsing University, Taichung, 402, China

    C. C. Young

Authors
  1. P. Priyanka
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  2. A. B. Arun
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  3. C. C. Young
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  4. P. D. Rekha
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Corresponding author

Correspondence to P. D. Rekha.

Additional information

This work was financially supported by the India and author Priyanka P acknowledges the Yenepoya University Junior research fellowship. The authors thank BRNS (2013/34/19/BRNS/1210).

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Priyanka, P., Arun, A.B., Young, C.C. et al. Prospecting exopolysaccharides produced by selected bacteria associated with marine organisms for biotechnological applications. Chin J Polym Sci 33, 236–244 (2015). https://doi.org/10.1007/s10118-015-1581-7

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  • DOI: https://doi.org/10.1007/s10118-015-1581-7

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