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Photobiomodulation by a new optical fiber device: analysis of the in vitro impact on proliferation/migration of keratinocytes and squamous cell carcinomas cells stressed by X-rays

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Abstract

Photobiomodulation-based (PBM-based) therapies show promising results in mucositis and dermatitis treatment by stimulating wound healing mechanisms such as cell proliferation and migration. The aim of the present study is to investigate the in vitro effects of CareMin650 on the proliferation and migration of two different types of cells, namely cancer and non-cancer cells, with or without X-ray radiation. Study design used PBM through a combination of 0-3-6 J/cm2 doses—with or without X-ray radiation—on the proliferation and migration capabilities of a keratinocyte cell line (HaCaT) and a squamous cell carcinoma line (SCC61). PBM is delivered by a new woven optical fiber device, namely CareMin650 prototype (light emission by LEDs (light-emitting diodes), peak at 660 nm, irradiance of 21.6 mW/cm2). The effectiveness of PBM to increase HaCaT proliferation and migration (with or without X-ray radiation) supports the capability of PBM to favor wound healing. It also highlights that PBM does not provide any anti-radiation effect to previously X-rays radiated SCC (p < 0.001). Such data supports the beneficial effect of PBM delivered by an optical fiber device to heal wounds, without promoting cancer development.

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HaCaT is a spontaneously transformed keratinocyte cell line from adult human skin. SCC is a head and neck squamous cell carcinoma (HNSCC) cell line, derived from a tongue cancer. These cell lines were obtained from the American Type Culture Collection (ATCC, USA).

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Acknowledgments

This work was conducted at the Faculty of Medicine Lyon Sud of Lyon 1 University (UMS2444/US8).

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Authors and Affiliations

  1. NeoMedLight SA, Villeurbanne, France

    Elodie Courtois, Fabrice Axisa, Pierre Saint-Girons, Laure Alston & Anne Visbecq

  2. Laboratoire de Radiobiologie Cellulaire et Moléculaire, Faculté de Médecine Lyon-Sud, Université Lyon 1, Oullins, France

    Jean-Baptiste Guy, Narimène Houmera, Claire Rodriguez-Lafrasse & Nicolas Magné

  3. Département de Radiothérapie, Institut de Cancérologie de la Loire, 108 bis, avenue Albert Raimond, BP 60008, 42270, Saint-Priest en Jarez cedex, France

    Jean-Baptiste Guy, Narimène Houmera & Nicolas Magné

  4. Centre de Haute Energie, Nice, France

    René-Jean Bensadoun

Authors
  1. Elodie Courtois
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Contributions

Conceptualization: Fabrice Axisa, Elodie Courtois, Laure Alston, Pierre Saint-Girons; methodology: Jean-Baptiste Guy, Elodie Courtois, René-Jean Bensadoun; formal analysis and investigation: Elodie Courtois, Jean-Baptiste Guy, Narimène Houmera; writing–original draft preparation: Elodie Courtois; writing–review and editing: Fabrice Axisa, Nicolas Magné, Anne Visbecq; resources: full name; supervision: Claire Rodriguez-Lafrasse, Pierre Saint-Girons.

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Correspondence to Nicolas Magné.

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Elodie Courtois (R&D Engineer), Fabrice Axisa (R&D Director), Pierre Saint-Girons (CEO), and Anne Visbecq (CMO) are employees of NeoMedLight. The authors declare that they have no other conflict of interest. The authors had full control of the primary data and agree to allow review of said data, if requested by the journal.

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Courtois, E., Guy, JB., Axisa, F. et al. Photobiomodulation by a new optical fiber device: analysis of the in vitro impact on proliferation/migration of keratinocytes and squamous cell carcinomas cells stressed by X-rays. Lasers Med Sci 36, 1445–1454 (2021). https://doi.org/10.1007/s10103-020-03185-x

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