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Comparative effects of photobiomodulation therapy at wavelengths of 660 and 808 nm on regeneration of inferior alveolar nerve in rats following crush injury

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Abstract

The aim of the present study was to investigate the therapeutic effects of 660-nm and 880-nm photobiomodulation therapy (PBMT) following inferior alveolar nerve (IAN) crush injury. Following the nerve crush injuries of IAN, 36 Wistar rats were randomly divided into three groups as follows: (1) control, (2) 660-nm PBMT, and (3) 808-nm PBMT (GaAlAs laser, 100 J/cm2, 70 mW, 0.028-cm2 beam). PBMT was started immediately after surgery and performed once every 3 days during the postoperative period. At the end of the 30-day treatment period, histopathological and histomorphometric evaluations of tissue sections were made under a light and electron microscope. The ratio of the inner axonal diameter to the total outer axonal diameter (g-ratio) and the number of axons per square micrometer were evaluated. In the 808-nm PBMT group, the number of nerve fibers with suboptimal g-ratio ranges of 0–0.49 (p < 0.001) is significantly lower than expected, which indicates better rate of myelinization in the 808-nm PBMT group. The number of axons per square micrometer was significantly higher in the 808-nm PBMT group when compared with the control (p < 0.001) and 660-nm PBMT group (p = 0.010). The data and the histopathological investigations suggest that the PBMT with the 808-nm wavelength along with its settings was able to enhance IAN regeneration after nerve crush injury.

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Funding

This research was funded by Baskent University Research Fund (Grand Number D-DA 14/06).

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Bezmialem Vakif University, Adnan Menderes Bulvarı, Vatan Caddesi, 34093 Fatih, Istanbul, Turkey

    Nurettin Diker

  2. Private Practice, Cankaya Mahallesi, Cankaya Caddesi No. 10/3 Cankaya, Ankara, Turkey

    Duygu Aytac

  3. Department of Histology and Embryology, Baskent University, Fatih Sultan Mahallesi, Eskişehir Yolu 18 km 06790 Etimesgut, Ankara, Turkey

    Fatma Helvacioglu

  4. Private Practice, Drs. Nicolas & ASP Dental Center, Jumeirah Beach Road, Dubai, United Arab Emirates

    Yener Oguz

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  1. Nurettin Diker
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  2. Duygu Aytac
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  3. Fatma Helvacioglu
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  4. Yener Oguz
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Correspondence to Nurettin Diker.

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All procedures performed in the present study, involving animals, were approved by the Institutional Review Board and Ethical Committee of Baskent University for experimental research on animals.

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Diker, N., Aytac, D., Helvacioglu, F. et al. Comparative effects of photobiomodulation therapy at wavelengths of 660 and 808 nm on regeneration of inferior alveolar nerve in rats following crush injury. Lasers Med Sci 35, 413–420 (2020). https://doi.org/10.1007/s10103-019-02838-w

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  • DOI: https://doi.org/10.1007/s10103-019-02838-w

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