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Effects of 915 nm laser irradiation on human osteoblasts: a preliminary in vitro study

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Abstract

Photobiomodulation (PBM) is a non-invasive treatment that uses laser or led devices making its effects a response to light and not to heat. The possibility of accelerating dental implant osteointegration and orthodontic movements and the need to treat refractory bone lesions, such as bisphosphonate related osteonecrosis of the jaws, has led researchers to consider the effects of PBM on bone for dentistry purposes. The aim of our study was to investigate the effects of 915 nm light supplied with a GaAs diode laser on human osteoblasts in vitro. Osteoblasts were isolated from mandibular cortical bone of a young healthy donor. The irradiation parameters were as follows: doses = 5, 15 and 45 J/cm2; power densities = 0.12 and 1.25 W/cm2; and irradiation times = 41.7, 125 and 375 s. We performed one irradiation per day for 3 and 6 days to study proliferation and differentiation, respectively. Microscopic analysis showed a greater amount of bone nodules in samples treated with 5 J/cm2 and 0.12 W/cm2 compared to controls (56.00 ± 10.44 vs 19.67 ± 7.64, P = 0.0075). Cell growth and quantification of calcium deposition did not show any differences when comparing irradiated and non-irradiated samples. Photobiomodulation, with the parameters investigated in the present study, positively modulated the mineralization process in human osteoblasts, inducing the formation of a greater amount of bone nodules, but did not increase cell proliferation.

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Author information

Authors and Affiliations

  1. Unit of Oral Pathology and Laser-assisted Oral Surgery; Department of Medicine and Surgery, University of Parma, Parma, Italy

    Giovanni Mergoni & Paolo Vescovi

  2. Unit of Anatomy Histology and Embryology, Department of Medicine and Surgery, University of Parma, Parma, Italy

    Silvana Belletti & Rita Gatti

  3. Unità Operativa di Patologia Clinica, Ospedale di Vaio, Fidenza, Italy

    Jacopo Uggeri

  4. Department of Dental Sciences, Faculty of Medicine, University of Liège, Liege, Belgium

    Samir Nammour

Authors
  1. Giovanni Mergoni
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  2. Paolo Vescovi
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  3. Silvana Belletti
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  4. Jacopo Uggeri
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  5. Samir Nammour
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  6. Rita Gatti
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Correspondence to Giovanni Mergoni.

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The authors declare that they have no conflict of interest.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Mergoni, G., Vescovi, P., Belletti, S. et al. Effects of 915 nm laser irradiation on human osteoblasts: a preliminary in vitro study. Lasers Med Sci 33, 1189–1195 (2018). https://doi.org/10.1007/s10103-018-2453-5

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  • DOI: https://doi.org/10.1007/s10103-018-2453-5

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