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Surface roughness and bacterial adhesion on root dentin treated with diode laser and conventional desensitizing agents

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Abstract

The treatments for dentin hypersensitivity (DH) may change the surface roughness of the root dentin, which can lead to biofilm accumulation, increasing the risk of root caries. The aim was to compare the surface roughness of root dentin after different treatments of DH and the biofilm formation on those surfaces. After initial surface roughness (Sa) assessment, 50 bovine root fragments received the following treatments (n = 10): G 1—no treatment; G2—5% sodium fluoride varnish; G3—professional application of a desensitizing dentifrice; G4—toothbrushing with a desensitizing dentifrice; and G5—diode laser application (908 nm; 1.5 W, 20 s). The Sa was reevaluated after treatments. Afterward, all samples were incubated in a suspension of Streptococcus mutans at 37 °C for 24 h. The colony-forming units (CFU) were counted using a stereoscope, and the results were expressed in CFU/mL. The one-way ANOVA and the Tukey’s tests compared the roughness data and the results obtained on the bacterial adhesion test (α = 5%). G2 (2.3 ± 1.67%) showed similar Sa variation than G1 (0.25 ± 0.41%) and G5 (5.69 ± 0.99%), but different from group G3 (9.05 ± 2.39%). Group 4 showed the highest Sa variation (30.02 ± 3.83%; p < 0.05). Bacterial adhesion was higher in G4 (2208 ± 211.9), suggesting that bacterial growth is greater on rougher surfaces. The diode laser and the conventional treatments for DH may change the surface roughness of the root dentin, but only brushing with desensitizing dentifrice induced a higher bacteria accumulation on root dentin surface.

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Authors and Affiliations

  1. Department of Clinical Dentistry, School of Dentistry, Uberaba University, Av. Nene Sabino, 1801 Sala 2D04, Uberaba, MG, 38 055 500, Brazil

    Maiza S. Cury, Michelle G. D. Campos & Vinicius R. Geraldo-Martins

  2. Biopathology Division, Uberaba University, Av. Nene Sabino, 1801 Sala 2D04, Uberaba, MG, 38 055 500, Brazil

    Camilla B. Silva & Ruchele D. Nogueira

  3. Department of Restorative Dentistry, Ribeirao Preto Dental School, São Paulo University, Avenida do Cafe, s/n, Ribeirao Preto, SP, 14040 904, Brazil

    Regina G. Palma-Dibb

Authors
  1. Maiza S. Cury
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  2. Camilla B. Silva
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  3. Ruchele D. Nogueira
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  4. Michelle G. D. Campos
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  5. Regina G. Palma-Dibb
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  6. Vinicius R. Geraldo-Martins
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Correspondence to Vinicius R. Geraldo-Martins.

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Cury, M.S., Silva, C.B., Nogueira, R.D. et al. Surface roughness and bacterial adhesion on root dentin treated with diode laser and conventional desensitizing agents. Lasers Med Sci 33, 257–262 (2018). https://doi.org/10.1007/s10103-017-2356-x

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  • DOI: https://doi.org/10.1007/s10103-017-2356-x

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