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Picosecond pulsed infrared laser tuned to amide I band dissociates polyglutamine fibrils in cells

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An Erratum to this article was published on 10 August 2016

Abstract

Amyloid fibrils are causal substances for serious neurodegenerative disorders and amyloidosis. Among them, polyglutamine fibrils seen in multiple polyglutamine diseases are toxic to neurons. Although much efforts have been made to explore the treatments of polyglutamine diseases, there are no effective drugs to block progression of the diseases. We recently found that a free electron laser (FEL), which has an oscillation wavelength at the amide I band (C = O stretch vibration mode) and picosecond pulse width, was effective for conversion of the fibril forms of insulin, lysozyme, and calcitonin peptide into their monomer forms. However, it is not known if that is also the case in polyglutamine fibrils in cells. We found in this study that the fibril-specific β-sheet conformation of polyglutamine peptide was converted into nonfibril form, as evidenced by the infrared microscopy and scanning-electron microscopy after the irradiation tuned to 6.08 μm. Furthermore, irradiation at this wavelength also changed polyglutamine fibrils to their nonfibril state in cultured cells, as shown by infrared mapping image of protein secondary structure. Notably, infrared thermography analysis showed that temperature increase of the cells during the irradiation was within 1 K, excluding thermal damage of cells. These results indicate that the picosecond pulsed infrared laser can safely reduce amyloid fibril structure to the nonfibril form even in cells.

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Abbreviations

DMSO:

Dimethyl sulfoxide

FEL:

Free electron laser

IRM:

Infrared microscopy

OM:

Optical microscopy

PBS:

Phosphate-buffered saline

SCA:

Spinocerebellar ataxia

SEM:

Scanning electron microscopy

TAMRA:

Tetra-methyl rhodamine

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Acknowledgments

We are grateful to the staff of IR-FEL Research Center at Tokyo University of Science for kindly providing the beam time. This work was supported in part by the Open Advanced Research Facilities Initiative and Photon Beam Platform Project of the Ministry of Education, Culture, Sport, Science and Technology, Japan

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Authors and Affiliations

  1. IR Free Electron Laser Research Center, Research Institute for Science and Technology, Organization for Research Advancement, Tokyo University of Science, 2641, Yamazaki, Noda, Chiba, 278-8510, Japan

    Takayasu Kawasaki, Gaku Ohori, Tomoyuki Chiba & Koichi Tsukiyama

  2. Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, 3-39-22, showa-machi, Maebashi, Gunma, 371-8511, Japan

    Kazuhiro Nakamura

Authors
  1. Takayasu Kawasaki
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  2. Gaku Ohori
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  3. Tomoyuki Chiba
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  4. Koichi Tsukiyama
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  5. Kazuhiro Nakamura
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Correspondence to Kazuhiro Nakamura.

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Kawasaki, T., Ohori, G., Chiba, T. et al. Picosecond pulsed infrared laser tuned to amide I band dissociates polyglutamine fibrils in cells. Lasers Med Sci 31, 1425–1431 (2016). https://doi.org/10.1007/s10103-016-2004-x

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  • DOI: https://doi.org/10.1007/s10103-016-2004-x

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