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Gingival tissue healing following Er:YAG laser ablation compared to electrosurgery in rats

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Abstract

The erbium-doped yttrium aluminum garnet (Er:YAG) laser is currently used for periodontal soft tissue management with favorable outcomes. However, the process of wound healing after Er:YAG laser (ErL) treatment has not been fully elucidated yet. The aim of this study was to investigate the gingival tissue healing after ErL ablation in comparison with that after electrosurgery (ElS). Gingival defects were created in 28 rats by ablation with ErL irradiation or ElS. The chronological changes in wound healing were evaluated using histological, histometrical, and immunohistochemical analyses. The ErL-ablated gingival tissue revealed much less thermal damage, compared to the ElS. In the ElS sites, the postoperative tissue destruction continued due to thermal damage, while in the ErL sites, tissue degradation was limited and the defects were re-epithelialized early. Heat shock protein (Hsp) 72/73 expression was detected abundantly remote from the wound in the ElS, whereas it was slightly observed in close proximity to the wound in the ErL sites. Hsp47 expression was observed in the entire connective tissue early in the wound healing and was found limited in the wound area later. This phenomenon proceeded faster in the ErL sites than in the ElS sites. Expression of proliferating cell nuclear antigen (PCNA) persisted in the epithelial tissue for a longer period in the ElS than that in the ErL. The ErL results in faster and more favorable gingival wound healing compared to the ElS, suggesting that the ErL is a safe and suitable tool for periodontal soft tissue management.

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Acknowledgments

This study was supported in part by a grant from the Global Center of Excellence (GCOE) Program for International Research Center for Molecular Science in Tooth and Bone Diseases at Tokyo Medical and Dental University from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by a Grant-in-Aid for Scientific Research (C) (22592308) to A.A. from the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan

    Masanori Sawabe, Akira Aoki, Mayumi Ogita & Yuichi Izumi

  2. Department of Nanomedicine (DNP), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan

    Motohiro Komaki & Kengo Iwasaki

Authors
  1. Masanori Sawabe
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  2. Akira Aoki
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  3. Motohiro Komaki
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  4. Kengo Iwasaki
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  5. Mayumi Ogita
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  6. Yuichi Izumi
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Correspondence to Akira Aoki or Motohiro Komaki.

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Sawabe, M., Aoki, A., Komaki, M. et al. Gingival tissue healing following Er:YAG laser ablation compared to electrosurgery in rats. Lasers Med Sci 30, 875–883 (2015). https://doi.org/10.1007/s10103-013-1478-z

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  • DOI: https://doi.org/10.1007/s10103-013-1478-z

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