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Effects of low-power LED and therapeutic ultrasound in the tissue healing and inflammation in a tendinitis experimental model in rats

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Abstract

This work evaluated the anti-inflammatory response of low-power light-emitting diode (LED) and ultrasound (US) therapies and the quality and rapidness of tendon repair in an experimental model of tendinitis, employing histomorphometry and Raman spectroscopy. Tendinitis was induced by collagenase into the right tendon of 35 male Wistar rats with an average weight of 230 g. The animals were randomly separated into seven groups of five animals each: tendinitis without treatment—control (TD7 and TD14, where 1 and 2 indicated sacrifice on the 7th and 14th day, respectively), tendinitis submitted to US therapy (US7 and US14) and tendinitis submitted to LED therapy (LED7 and LED14). Contralateral tendons of the TD group at the 14th day were used as the healthy group (H). US treatment was applied in pulsed mode at 10 %, 1 MHz frequency, 0.5 W/cm2, 120 s. LED therapy parameters were 4 J/cm2, 120 s, daily dose at the same time and same point. Sacrifice was performed on the 7th or 14th day. Histomorphometric analysis showed lower number of fibroblasts on the 14th day of therapy for the US-treated group, compared to the TD and LED, indicating lower tissue inflammation. Raman showed that the LED group had an increase in the amount of collagen I and III from the 7th to the 14th day, which would indicate more organized fibers and a better quality of the healing, and US showed lower collagen I synthesis in the 14th day compared to H, indicating a lower tissue reorganization.

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Acknowledgments

L. Silveira, Jr. acknowledges National Counsel of Technological and Scientific Development (CNPq) for the Productivity Fellowship (no. 309209/2011-0).

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Authors and Affiliations

  1. Biomedical Engineering Institute, Universidade Camilo Castelo Branco–UNICASTELO, Parque Tecnológico de São José dos Campos, Estr. Dr. Altino Bondesan, 500, São José dos Campos, São Paulo, Brazil, 12247-016

    Manoel de Jesus Moura Júnior & Landulfo Silveira Jr.

  2. Faculdade Santo Agostinho–FSA, Av. Valter Alencar, 665, Teresina, Piauí, Brazil, 64019-625

    Manoel de Jesus Moura Júnior, Janderson Pereira de Carvalho & José Mário Nunes da Silva

  3. Institute of Research and Development, Universidade do Vale do Paraíba–UNIVAP, Av. Shishima Hifumi, 2911, São José dos Campos, São Paulo, Brazil, 12244-000

    Emilia Ângela Loschiavo Arisawa & Airton Abrahão Martin

  4. Center of Health Sciences, Universidade Estadual do Piauí–UESPI, Rua Olavo Bilac, 2335, Teresina, Piauí, Brazil, 64001-280

    José Figueiredo Silva

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  1. Manoel de Jesus Moura Júnior
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  2. Emilia Ângela Loschiavo Arisawa
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  3. Airton Abrahão Martin
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  4. Janderson Pereira de Carvalho
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  5. José Mário Nunes da Silva
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  6. José Figueiredo Silva
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  7. Landulfo Silveira Jr.
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Correspondence to Manoel de Jesus Moura Júnior.

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Moura Júnior, M.J., Arisawa, E.Â.L., Martin, A.A. et al. Effects of low-power LED and therapeutic ultrasound in the tissue healing and inflammation in a tendinitis experimental model in rats. Lasers Med Sci 29, 301–311 (2014). https://doi.org/10.1007/s10103-013-1327-0

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  • DOI: https://doi.org/10.1007/s10103-013-1327-0

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