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Photosensitizing and radiosensitizing effects of mitoxantrone: combined chemo-, photo-, and radiotherapy of DFW human melanoma cells

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Abstract

This study evaluated the effects of mitoxantrone (MX), an antitumor agent, as a sensitizer to both photodynamic and radiation therapy in DFW human melanoma cells. Cells were incubated with MX at different concentrations for 90 min and then exposed to non-coherent light at different fluence rates and/or X-ray ionizing radiation at different dose rates. Combinatorial effects of this chemo-, photo-, and radiotherapy were also evaluated. MX had no significant effects on viability at moderate doses but had a strong cytotoxic effect on cancer cells when used as a photosensitizer. MX also acted as a potent radiosensitizer. We observed a dose-dependent effect on cell viability in cells exposed to MX in combination with phototherapy and radiotherapy. Strong synergistic effects were observed for combinations of two or more treatment methods, which, in some cases, induced complete cell death. Thus, a combination of ionizing radiation with MX-mediated photodynamic therapy could serve as a new method for cancer therapy with fewer adverse side effects.

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Acknowledgments

This study was supported by a grant from the Mashhad University of Medical Sciences, Mashhad, Iran.

Conflict of interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Department of Medical Physics, Research Center of Medical Physics, Mashhad University of Medical Sciences, Mashhad, Iran

    Ameneh Sazgarnia & Mohammad Hossein Bahreyni-Toosi

  2. Department of Medical Physics, Tehran University of Medical Sciences, Tehran, Iran

    Ali Reza Montazerabadi

  3. Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, P.O. Box 48175-861, 18 Kilometer Farahabad Rd, Sari, Iran

    Amirhossein Ahmadi

Authors
  1. Ameneh Sazgarnia
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  2. Ali Reza Montazerabadi
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  3. Mohammad Hossein Bahreyni-Toosi
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  4. Amirhossein Ahmadi
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Correspondence to Amirhossein Ahmadi.

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Sazgarnia, A., Montazerabadi, A.R., Bahreyni-Toosi, M.H. et al. Photosensitizing and radiosensitizing effects of mitoxantrone: combined chemo-, photo-, and radiotherapy of DFW human melanoma cells. Lasers Med Sci 28, 1533–1539 (2013). https://doi.org/10.1007/s10103-013-1275-8

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  • DOI: https://doi.org/10.1007/s10103-013-1275-8

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