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A quantitative diagnostic method for oral mucous precancerosis by Rose Bengal fluorescence spectroscopy

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Abstract

A novel in vivo fluorescence spectroscopic diagnostic method has been developed in an animal model to make a quantified precancer diagnosis. In the study, 40 golden hamsters were randomly divided into four groups (groups A, B, C, and D), with group A being the control group and the other three groups being inducted at different precancer stages. A 1% Rose Bengal (RB) solution was used for the fluorescence spectroscopic diagnosis. A parameter K defined as K = IRB/Iauto was introduced to reflect the amount of RB in the tissue, where IRB and Iauto represent the fluorescence peak intensity of the RB in the tissue and the autofluorescence intensity of tissue at 580 nm, respectively. The average K values of the four groups were calculated and statistically analyzed by analysis of variance (ANOVA), which revealed statistically significant differences within each group as well as between groups (p < 0.001). After analysis by Clementine 11.1 C&R Tree modeling (CART), the following diagnostic criteria were set: normal, K ≤8.91; simple hyperplasia, 8.91 < K ≤41.92; mild dysplasia, 41.92 < K ≤ 70.79; moderate and severe dysplasia, K >70.79. The sensitivity and specificity to detect precancerous lesions compared with scalpel biopsy were calculated. The results of this study showed that the spectrofluorometric method mediated by RB could accurately discriminate different precancer stages.

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Acknowledgments

This research was supported by the scientific research fund from the Department of Education, Heilongjiang Province (No: 11541148) and the research fund from the First Affiliated Hospital of Harbin Medical University (2011BS006).

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Authors and Affiliations

  1. Department of Preventive Dentistry, The First Affiliated Hospital, Harbin Medical University, Harbin, 150001, People’s Republic of China

    Lei Zhang & Jinna Shi

  2. Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, 150001, People’s Republic of China

    Liangjia Bi & Jiang Lin

  3. Condensed Matter Science and Technology Institute, Harbin Institute of Technology, Harbin, 150080, People’s Republic of China

    Zhiguo Zhang & Wenwu Cao

  4. Materials Research Institute, The Pennsylvania State University, University Park, PA, 16802, USA

    Wenwu Cao

  5. Department of Peridont & Oral Med, The Stomatology Hospital & School, Wuhan University, Wuhan, 430079, People’s Republic of China

    Chengzhang Li & Jiarui Bi

  6. Department of Peridontology, The Stomatology Hospital, Harbin Medical University, Harbin, 150001, People’s Republic of China

    Yang Yu

Authors
  1. Lei Zhang
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  2. Liangjia Bi
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  3. Jinna Shi
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  4. Zhiguo Zhang
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  5. Wenwu Cao
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  6. Jiang Lin
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  7. Chengzhang Li
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  8. Jiarui Bi
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  9. Yang Yu
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Corresponding author

Correspondence to Liangjia Bi.

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Lei Zhang and Jinna Shi contributed equally to this work.

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Zhang, L., Bi, L., Shi, J. et al. A quantitative diagnostic method for oral mucous precancerosis by Rose Bengal fluorescence spectroscopy. Lasers Med Sci 28, 241–246 (2013). https://doi.org/10.1007/s10103-012-1054-y

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  • DOI: https://doi.org/10.1007/s10103-012-1054-y

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