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Evaluation of the osteogenic effect of low-level laser therapy (808 nm and 660 nm) on bone defects induced in the femurs of female rats submitted to ovariectomy

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Abstract

The present study aimed to evaluate the effects of LLLT (660- and 808-nm wavelengths) on the process of repairing bone defects induced in the femurs of female rats submitted to ovariectomy. Bilateral ovariectomies were performed on 18 female Wistar rats, which were divided into control and irradiated groups after the digital analysis of bone density showed decreased bone mass and after standardized drilling of the femurs. The irradiated groups received 133 J/cm2 of AsGaAl (660-nm) and InGaAlP (880-nm) laser radiation. The animals were euthanized on days 14 and 21 after the bone defects were established. Detailed descriptive histological evaluations were performed, followed by semi-quantitative histomorphometry. The results from days 14 and 21 showed that the irradiated groups presented increased density of osteoblasts, fibroblasts, and immature osteocytes on the tissue surface compared with the control (non-irradiated) groups (p < 0.05). Additionally, inflammatory infiltrate evaluations showed that LLLT decreased the accumulation of leukocytes when compared to the control treatment (p < 0.05). We concluded that, in our experimental model, both wavelengths (660-nm and 880-nm) inhibited the inflammatory process and induced the proliferation of cells responsible for bone remodeling and repair.

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Authors and Affiliations

  1. Postgraduate Program in Health and Development in the West Central Region, Federal University of Mato Grosso do Sul, Campo Grande, Brazil

    Rodrigo Ré Poppi, Anísio Lima Da Silva, Renato Silva Nacer & Paulo de Tarso Camilo Carvalho

  2. Department of Physiotherapy, University for the Development of the State and Pantanal Region (UNIDERP), Campo Grande, MS, Brazil

    Renato Silva Nacer

  3. Chronic Obstructive Pulmonary Disease and Asthma Research Group, Department of Pneumology, University Hospital Freiburg, Freiburg, Germany

    Rodolfo Paula Vieira

  4. Rehabilitation Sciences Master’s Program, University Nove de Julho-UNINOVE, São Paulo, SP, Brazil

    Luis Vicente Franco de Oliveira, Newton Santos de Faria Júnior & Paulo de Tarso Camilo Carvalho

  5. Rua Dr. Raul Guedes de Melo N. 57, Campinas, SP, Brazil

    Paulo de Tarso Camilo Carvalho

Authors
  1. Rodrigo Ré Poppi
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  2. Anísio Lima Da Silva
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  3. Renato Silva Nacer
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  4. Rodolfo Paula Vieira
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  5. Luis Vicente Franco de Oliveira
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  6. Newton Santos de Faria Júnior
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  7. Paulo de Tarso Camilo Carvalho
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Correspondence to Paulo de Tarso Camilo Carvalho.

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Ré Poppi, R., Da Silva, A.L., Nacer, R.S. et al. Evaluation of the osteogenic effect of low-level laser therapy (808 nm and 660 nm) on bone defects induced in the femurs of female rats submitted to ovariectomy. Lasers Med Sci 26, 515–522 (2011). https://doi.org/10.1007/s10103-010-0867-9

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  • DOI: https://doi.org/10.1007/s10103-010-0867-9

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