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Histological examination of experimentally infected root canals after preparation by Er:YAG laser irradiation

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Abstract

The influence of Er:YAG laser irradiation on periodontal tissues along the root surface and apical region during root canal preparation was histologically evaluated using experimentally infected root canals of rats. Eighty experimentally mesial infected root canals of mandibular first molars in rats were divided into four groups. In three groups, root canals were irradiated using an Er:YAG laser at 2 Hz with 34, 68, or 102 mJ/pulse for 30 s. Non-irradiated canals served as controls. The influence of laser irradiation on periodontal tissues along the root surface and apical area was evaluated histologically under light microscopy at 0 (immediately after), 1, 2, 4, and 8 weeks after irradiation. At all periods, no inflammation or resorption on the root surfaces caused by laser irradiation was observed in any cases in the control or 34 mJ/pulse-irradiated groups. However, mild to severe inflammation with resorption of root surfaces was observed in some cases in the 68- and 102-mJ/pulse-irradiated groups. No significant difference was apparent between control and laser-irradiated groups at the apical area for all experimental periods (p > 0.05). These results suggest that thermal influences on periodontal tissues of experimentally infected root canals during root canal preparation by Er:YAG laser irradiation are minimal if appropriate parameters are selected. Er:YAG laser irradiation is thus a potential therapy for human infected root canals.

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Acknowledgments

This study was supported in part by a Grant-in-Aid for Scientific Research (11671902) from the Japan Society for the Promotion of Science (JSPS) and also by MEXT. HITEKU (2005–2009).

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Authors and Affiliations

  1. Division of Endodontics, Department of Conservative Dentistry, Ohu University School of Dentistry, 31-1 Misumido, Tomita-machi, Koriyama City, Fukushima, 963-8611, Japan

    Yuichi Kimura, Michihiko Tanabe, Hiroaki Imai & Yoshikazu Amano

  2. Department of Endodontics, Showa University Dental Hospital, 2-1-1 Kitasenzoku, Ohta-ku, Tokyo, 145-8515, Japan

    Yoshiko Masuda & Yoshishige Yamada

Authors
  1. Yuichi Kimura
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  2. Michihiko Tanabe
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  3. Hiroaki Imai
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  4. Yoshikazu Amano
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  5. Yoshiko Masuda
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  6. Yoshishige Yamada
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Correspondence to Yuichi Kimura.

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Kimura, Y., Tanabe, M., Imai, H. et al. Histological examination of experimentally infected root canals after preparation by Er:YAG laser irradiation. Lasers Med Sci 26, 749–754 (2011). https://doi.org/10.1007/s10103-010-0804-y

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  • DOI: https://doi.org/10.1007/s10103-010-0804-y

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