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Low-level laser therapy (LLLT) attenuates RhoA mRNA expression in the rat bronchi smooth muscle exposed to tumor necrosis factor-α

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Abstract

Low-level laser therapy (LLLT) has been found to produce anti-inflammatory effects in a variety of disorders. Bronchial smooth muscle (BSM) hyperreactivity is associated with increased Ca+2 sensitivity and increased RhoA mRNA expression. In the current study, we investigated if LLLT could reduce BSM contraction force and RhoA mRNA expression in tumor necrosis factor-α (TNF-α)-induced BSM hyperreactivity. In the study, 112 male Wistar rats were divided randomly into 16 groups, and BSM was harvested and suspended in TNF-α baths for 6 and 24 h, respectively. Irradiation with LLLT was performed with a wavelength of 660 nm for 42 s with a dose of 1.3 J/cm2. This LLLT dose was administered once in the 6-h group and twice in the 24-h group. LLLT significantly decreased contraction force in BSM at 6 h (TNF-α + LLLT: 11.65 ± 1.10 g/100 mg of tissue) (F = 3115) and at 24 h (TNF-α + LLLT: 14.15 ± 1.1 g/100 mg of tissue) (F = 3245, p < 0.05) after TNF-α, respectively, when compared to vehicle-bathed groups (control). LLLT also significantly decreased the expression of RhoA mRNA in BSM segments at 6 h (1.22 ± 0.20) (F = 2820, p < 0.05) and 24 h (2.13 ± 0.20) (F = 3324, p < 0.05) when compared to BSM segments incubated with TNF-α without LLLT irradiation. We conclude that LLLT administered with this protocol, reduces RhoA mRNA expression and BSM contraction force in TNF-α-induced BSM hyperreactivity.

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Authors and Affiliations

  1. Instituto de Pesquisa e Desenvolvimento, IP&D, Av. Shishima Hifumi, 2911, São José dos Campos, CEP: 12244-000, São Paulo, SP, Brazil

    Flávia Mafra de Lima

  2. Department of Physiotherapy, Bergen University College, Møllendalsveien 9, 5009, Bergen, Norway

    Jan M. Bjordal

  3. Section of Physiotherapy Science, Inst. Public Health & Primary Health Care, University of Bergen, 5018, Bergen, Norway

    Jan M. Bjordal

  4. Rehabilitation Sciences Departament, Centro Universitário Nove de Julho - Uninove, Rua Vergueiro, 235, SP, Brazil

    Regiane Albertini

  5. Universidade Camilo Castelo Branco-UNICASTELO, Km 138, Rodovia Presidente Dutra, São José dos Campos, CEP: 12247-004, São Paulo, SP, Brazil

    Regiane Albertini & Flavio Aimbire

  6. Campus Centro Oeste, Divinópolis, Universidade Federal de São João Del Rei, CEP: Minas Gerais, MG, Brazil

    Fábio V. Santos

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  1. Flávia Mafra de Lima
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Correspondence to Flavio Aimbire.

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de Lima, F.M., Bjordal, J.M., Albertini, R. et al. Low-level laser therapy (LLLT) attenuates RhoA mRNA expression in the rat bronchi smooth muscle exposed to tumor necrosis factor-α. Lasers Med Sci 25, 661–668 (2010). https://doi.org/10.1007/s10103-010-0766-0

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