Abstract
The capacity of photo-sensitizers, used in combination with laser light to kill micro-organisms has been demonstrated in different studies. Photo-activated disinfection (PAD) has been introduced in periodontology as an aid for disinfection of periodontal pockets. The aim of this study is to verify the harm for dental vitality of the use of PAD in periodontal pockets. Root canals of 24 freshly extracted human teeth where prepared using profiles up to a size of ISO #50 and filled with thermo-conductor paste. A silicon-based false gum was made in which a periodontal pocket was created and filled with photo-sensitizer phenothiazine chloride (phenothiazine-5-ium, 3.7-bis (dimethylamino)-, chloride). The external root surface was irradiated during 60 s with a 660-nm diode laser (output power: 20 mW; power density: 0.090 W/cm2; Energy density: 5.46 J/cm2) using a periodontal tip with a diameter of 1 mm and a length of 7 mm. Temperatures were recorded inside the root canal using a thermocouple. Measurements were recorded every second, starting at 10 s before lasering, during the irradiation and were continued for 150 s after the end of irradiation, and six measurements were done per tooth. An average temperature increase of 0.48 ± 0.11°C was recorded. Our results demonstrated that pulp temperature increase was lower than 3°C, which is considered to be harmless for pulp injury. Regarding pulp temperature increase, the use of PAD for disinfection of periodontal pockets can be considered as a safe procedure for dental vitality.
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References
Manson JD, Eley BP (1995) Outline of periodontics, 3rd edn. Wright, Oxford, UK
Miyazaki H, Pilot T, Leclercq MH, Barmes DE (1991) Profiles of periodontal conditions in adults measured by CPTIN. Int Dent J 41(2):67–73
Feres M, Haffajee AD, Goncalves C, Allard KA, Som S, Smith C, Goodson JM, Socransky SS (1999) Systemic doxycycline administration in the treatment of periodontal infection. II. Effect on antibiotic resistance of subgingival species. J Clin Periodontol 26:784–792
Chaves ES, Jeffcoat MK, Ryerson CC, Snyder B (2000) Persistent bacterial colonisation of Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans in periodontitis and its association with alveolar bone loss after 6 months of therapy. J Clin Periodontol 27:897–903. doi:10.1034/j.1600-051x.2000.027012897.x
Walker A, Ash MN (1976) A study of root planning by scanning electron microscopy. Dent Hyg (Chic) 50:109–114
Waerhang J (1978) Healing of dento-epithelial junction following subgingival plaque control. II; As observed on extracted teeth. J Periodontol 49:119–134
Cafesse RG, Sweeney PL, Smith BA (1986) Scaling and root planning with and without periodontal flap surgery. J Clin Periodontol 13:205–210. doi:10.1111/j.1600-051X.1986.tb01461.x
Buchanan SA, Robertson PB (1987) Calculus removal by scaling and root planning with and without surgical access. J Periodontol 58:159–163
Lee MT (2003) Photoactivated disinfection of E. Faecalis in root canals using lasers. The University of Queensland, MDSc. St. Lucia
Sarkar S, Wilson M (1993) Lethal photosensitisation of bacteria in subgingival plaque from patients with chronic periodontitis. J Periodont Res 28:204–210
Haas R, Baron M, Dortbudak O, Watzek G (2000) Lethal photosensitisation, autologous bone and e-PTFE membrane for the treatment of peri-implantitis: preliminary results. Laryngoscope 105:867–871
Odor TM, Chandler NP, Watson TF, Pitt Ford TR, McDonald F (1999) Laser light transmission in teeth: a study of the patterns in different species. Int Endodontic J 32:296–302
Komerik N, Wilson M (2002) Factors influencing the susceptibility of Gram-negative bacteria to toluidine blue-mediated lethal photosensitisation. J Appl Microbiol 92:618–623. doi:10.1046/j.1365-2672.2002.01567.x
Braun A, Dehn C, Krause F, Jepsen S (2008) Short-term clinical effects of adjunctive antimicrobial photodynamic therapy in periodontal treatment: a randomized clinical trial. J Clin Periodontol Oct 35(10):877–884
Christodoulides N, Nikolidakis D, Chondros P, Becker J, Schwarz F, Rössler R, Sculean A (2008) Photodynamic therapy as an adjunct to non-surgical periodontal treatment: a randomized controlled clinical trial. J Periodontol 79(9):1638–1644
Zach L, Cohen G (1965) Pulp response to externally applied heat. Oral Surg Oral Med Oral Pathol 19:515–530. doi:10.1016/0030-4220(65)90015-0
Slots J, Jorgensen MJ (2002) Effective, safe, practical and affordable periodontal antimicrobial therapy: where are we going, and are we there yet? J Periodontol 2000(28):298–312
Oosterwaal PJ, Mikx FH, Renggli HH (1990) Clearance of a topically applied fluorescein gel from periodontal pockets. J Clin Periodontol 17:613–615. doi:10.1111/j.1600-051X.1990.tb01681.x
Marsh P (2003) Plaque as a biofilm: pharmacological principles of drug delivery and action in the sub- and supragingival environment. Oral Dis 9(Suppl 1):16–22. doi:10.1034/j.1601-0825.9.s1.4.x
Bhatti M, MacRobert A, Meghji S, Henderson B, Wilson M (1998) A study of the uptake of toluidine blue O by Porphyromanos gingivalis and the mechanism of lethal photosensitization. Photochem Photobiol 68:370–376. doi:10.1111/j.1751-1097.1998.tb09694.x
Bhatti M, Macrobert A, Henderson B, Shepherd P, Cridland J, Wilson M (2000) Antibody targeted lethal photosensitization of Porphyromonas gingivalis. Antimicrob Agents Chemother 44:2615–2618. doi:10.1128/AAC.44.10.2615-2618.2000
Komerik N, Wilson M, Pool S (2000) The effect of photodynamic action on two virulence factors of Gram-negative bacteria 72:676–680
Komerik N, Nakanishi H, Macrobert AJ, Henderson B, Speight P, Wilson M (2003) In vivo killing of Porphyromonas gingivalis by toluidine blue-mediated photosensitization in an animal model. Antimicrob Agents Chemother 47:932–940. doi:10.1128/AAC.47.3.932-940.2003
Sarkar S, Wilson M (1993) Lethal photosensitization of bacteria in subgingival plaque from patients with chronic periodontitis. J Periodontal Res 28:204–210. doi:10.1111/j.1600-0765.1993.tb01070.x
Wilson M, Dobson J, Sarkar S (1993) Sensitization of periodontopathogenic bacteria to killing by light from a low-power laser. Oral Microbiol Immunol 8:182–187. doi:10.1111/j.1399-302X.1993.tb00663.x
Wilson M, Dobson J (1993) Lethal photosensitization of oral anaerobic bacteria. Clin Infect Dis 16(suppl 4):S414–S415
Wilson M, Sarkar S, Bulman JS (1993) Effect of blood on lethal photosensitization of bacteria in subgingival plaque from patients with chronic periodontitis. Lasers Med Sci 8:297–303. doi:10.1007/BF02547854
Wilson M (1994) Bactericidal effect of laser light and its potential use in the treatment of plaque-related diseases. Int Dent J 44:181–189
de Oliveira RR, Schwartz-Filho HO, Novaes AB Jr., Taba M Jr. (2007) Antimicrobial photodynamic therapy in the non-surgical treatment of aggressive periodontitis: a preliminary randomized controlled clinical study. J Periodontol 78(6):965–973. doi:10.1902/jop. 2007.060494
Komerik N, Curnow A, Macrobert AJ, Hopper C, Speight PM, Wilson M (2002) Fluorescence biodistribution and photosensitizing activity of toluidine blue O on rat buccal mucosa. Lasers Med Sci 17:86–92. doi:10.1007/s101030200015
O’Neill JF, Hope CK, Wilson M (2002) Oral bacteria in multi-species biofilms can be killed by red light in the presence of toluidine blue. Lasers Surg Med 31:86–90. doi:10.1002/lsm.10087
Wilson M, Wilson H (1997) Laser treatment, US patent 5,611,793
Walsh LJ (2003) The current status of laser applications in dentistry. Aust Dent J 48:146–155. doi:10.1111/j.1834-7819.2003.tb00025.x
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El Yazami, H., Zeinoun, T., Bou Saba, S. et al. Pulp temperature increase during photo-activated disinfection (PAD) of periodontal pockets: an in vitro study. Lasers Med Sci 25, 655–659 (2010). https://doi.org/10.1007/s10103-009-0686-z
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DOI: https://doi.org/10.1007/s10103-009-0686-z